Westase（酵母細胞壁溶解酵素）

菌株	培地	培養条件	プロトプラスト調製条件
saccharomyces cerevisiae trigonopsis variabilis	YPG	25℃, 1 day Shake culture (reciprocate at 120 rpm)	0.5% Westase solution 0.5 M Na-tartrate McIlvain buffer (pH6.0) 30℃, Reciprocal shaking, 1-2 hr
lipomyces starkeyi candida utilis	YPG	25℃, 1 day Shake culture (reciprocate at 120 rpm)	0.5% Westase solution 0.4 M Na-tartrate McIlvain buffer (pH6.0) 30℃, Reciprocal shaking, 1-2 hr
shizosaccharomyces pombe hansenula mrakii kluyveromyces lactis pichia anomala filobasidium floriforme candida colliculosa	YPG	25℃, 1 day Shake culture (reciprocate at 120 rpm)	0.5% Westase solution 0.4 M Na-tartrate McIlvain buffer (pH6.0) 30℃, Reciprocal shaking, 3-4 hr
kloeckera apiculata cryptococcus albidus	YPG	25℃, 1 day Shake culture (reciprocate at 120 rpm)	0.5% Westase solution 0.3 M Na-tartrate McIlvain buffer (pH6.0) 30℃, Reciprocal shaking, 1-2 hr
ustilago maydis graphiola phoenicis brettanomyces bruxellensis phaffia rhodozyma	YPG	25℃, 2 day Shake culture (reciprocate at 120 rpm)	0.5% Westase solution 0.5 M Na-tartrate McIlvain buffer (pH6.0) 30℃, Reciprocal shaking, 1-2 hr
tremella mesenterica	YPG	25℃, 2 day Shake culture (reciprocate at 120 rpm)	0.5% Westase solution 0.4 M Na-tartrate McIlvain buffer (pH6.0) 30℃, Reciprocal shaking, 1-2 hr

YPG培地: 0.5％酵母エキス、1.0％ポリペプトン、1.0％グルコース、0.1％ KH₂PO₄、0.05％ MgSO₄･7H₂O, pH5.5。
McIlvain Buffer: 0.1 Mクエン酸溶液と0.2 Mリン酸水素二ナトリウム溶液を約36.8：63.2で混合し、pH6.0に調整する。