2×Multiplex PCR Buffer(Mg2+, dNTP plus) | 25 μl |
Primer 1 (20 pmol/μl) | 0.5 μl |
Primer 2 (20 pmol/μl) | 0.5 μl |
Multiplex PCR Enzyme Mix | 0.25 μl |
template DNA | 適宜 |
dH2O | up to 50 μl |
94℃ | 30 sec. | ||
↓ | |||
94℃ | 30 sec. | 30~40 cycles | |
55~60℃ | 10~15 sec.* | ||
72℃ | 30 sec./kb |
94℃ | 30 sec. | ||
↓ | |||
94℃ | 30 sec. | 30~40 cycles | |
65℃ | 30 sec./kb |