* | R: | A or G | ||
Y: | C or T | D: | A or G or T | |
W: | A or T | H: | A or C or T | |
S: | G or C | |||
K: | G or T | N: | A or C or G or T | |
M: | A or C |
制限酵素 | 認識配列* | 切断後の 末端構造* |
対合末端を生じる 制限酵素 |
対合末端との ライゲーション後の配列* |
ライゲーション後の配列を 認識する制限酵素 |
Aat II | GACGT↓C | GACGT3' C 5' |
Aat II | GACGTC | Aat II, Hin1 I |
Acc I | GT↓MKAC | GT 3' CATC5' |
Acc I | GTAGAC | Acc I |
GT 3' CATA5' |
Acc I | GTATAC | Acc I,Bst1107 I | ||
GT 3' CAGC5' |
Acc I | GTCGAC | Acc I, Hinc II, Sal I, Taq I | ||
BspT104 I, Cla I, Taq I | GTCGA | Taq I | |||
Hap II | GTCGG | - | |||
Hin1 I | GTCGYC | - | |||
GT 3' CAGA5' |
Acc I | GTCTAC | Acc I | ||
Acc II | CG↓CG | CG3' GC5' |
Acc II, Nru I | CGCG | Acc II |
Bst1107 I | CGTAC | Afa I | |||
EcoR V | CGATC | Sau3A I | |||
Acc III | T↓CCGGA | T 3' |
Acc III, Aor13H I | TCCGGA | Acc III, Aor13H I, Hap II |
Ava I, BmeT110 I | TCCGGG | Bcn I, Hap II | |||
Cfr10 I | TCCGGY | Hap II | |||
Afa I | GT↓AC | GT3' CA5' |
Afa I, Sca I | GTAC | Afa I |
Nru I | CTCGA | Taq I | |||
Afl II | C↓TTAAG | C 3' GAATT5' |
Afl II | CTTAAG | Afl II, RspRS II |
Alu I | AG↓CT | AG3' TC5' |
Alu I, Pvu II | AGCT | Alu I |
Bst1107 I | AGTAC | Afa I | |||
EcoR V | AGATC | Sau3A I | |||
Aor13H I | T↓CCGGA | T 3' AGGCC5' |
Acc III, Aor13H I | TCCGGA | Acc III, Aor13H I,Hap II |
Ava I, BmeT110 I | TCCGGG | Bcn I, Hap II | |||
Cfr10 I | TCCGGY | Hap II | |||
Aor51H I | AGC↓GCT | AGC3' TCG5' |
Aor51H I | AGCGCT | Aor51H I,Hae II,Hha I |
Avi II | AGCGCA | Hha I | |||
Bst1107 I | AGCTAC | Alu I | |||
Apa I | GGGCC↓C | GGGCC3' C 5' |
Apa I, Ban II, Bsp1286 I | GGGCCC | Apa I, Ban II, BmgT120 I, Bsp1286 I, Cfr13 I, Hae III |
ApaL I | G↓TGCAC | G 3' CACGT5' |
ApaL I | GTGCAC | ApaL I,Bsp1286 I |
Ava I | C↓YCGRG | C 3' GGGCT5' |
Ava I, BmeT110 I | CCCGAG | Ava I, BmeT110 I |
C 3' GGGCC5' |
Acc III, Aor13H I, Cfr10 I | CCCGGH | Bcn I,Hap II | ||
Ava I, BmeT110 I | CCCGGG | Ava I, BmeT110 I, Bcn I,Hap II,Sma I | |||
C 3' GAGCT5' |
Ava I, BmeT110 I, Xho I | CTCGAG | Ava I, BmeT110 I, Taq I,Xho I | ||
Sal I | CTCGAC | Taq I | |||
C 3' GAGCC5' |
Ava I, BmeT110 I | CTCGGG | Ava I, BmeT110 I | ||
Ava II (VpaK11B I) |
G↓GWCC | G 3' CCWG5' |
Ava II, BmgT120 I, Cfr13 I, Cpo I, EcoO109 I, VpaK11B I | GGWCC | Ava II, BmgT120 I, Cfr13 I, VpaK11B I |
Avi II | TGC↓GCA | TGC3' ACG5' |
Aor51H I | TGCGCT | Hha I |
Avi II | TGCGCA | Avi II,Hha I | |||
Bal I | TGG↓CCA | TGG3' ACC5' |
Bal I | TGGCCA | Bal I,Eae I,Hae III |
Bst1107 I | TGGTAC | Afa I | |||
EcoR V | TGGATC | Sau3A I | |||
Hae III, Stu I | TGGCC | Hae III |
制限酵素 | 認識配列* | 切断後の 末端構造* |
対合末端を生じる 制限酵素 |
対合末端との ライゲーション後の配列* |
ライゲーション後の配列を 認識する制限酵素 |
BamH I | G↓GATCC |
G 3' CCTAG5' |
BamH I | GGATCC | BamH I,Mfl I,Sau3A I |
Bgl II | GGATCT | Mfl I,Sau3A I | |||
Fba I, Sau3A I | GGATC | Sau3A I | |||
Mfl I | GGATCY | Mfl I,Sau3A I, Y=C:BamH I |
|||
Ban II | GRGCY↓C |
GAGCT3' C 5' |
Ban II, Bsp1286 I, Sac I | GAGCTC | Alu I,Apa I,Ban II,Bsp1286 I,Sac I |
GGGCC3' C 5' |
Apa I, Ban II, Bsp1286 I | GGGCCC | Apa I,Ban II, BmgT120 I, Bsp1286 I, Cfr13 I,Hae III | ||
Bbe I | GGCGC↓C |
GGCGC3' C 5' |
Bbe I | GGCGCC | Bbe I,Hae II,Hha I,Hin1 I |
Hae II | GGCGCY | Hha I Y=C:Bbe I, Hae II, Hin1 I |
|||
Bcn I | CC↓SGG |
CC 3' GGG5' |
Bcn I | CCCGG | Bcn I, Hap II |
Tth111 I | CCCNGTC | N=G:Bcn I, Hap II | |||
CC 3' GGC5' |
Bcn I | CCGGG | Bcn I, Hap II | ||
Tth111 I | CCGNGTC | N=A:Hinf I N=C:Acc II N=G:Bcn I,Hap II |
|||
Bgl II | A↓GATCT |
A 3' TCTAG5' |
BamH I | AGATCC | Mfl I, Sau3A I |
Bgl II | AGATCT | Bgl II, Mfl I, Sau3A I | |||
Fba I, Sau3A I | AGATC | Sau3A I | |||
Mfl I | AGATCY | Mfl I, Sau3A I Y=T:Bgl II |
|||
Bln I | C↓CTAGG | C 3' GGATC5' |
Bln I,EcoT14 I | CCTAGG | Bln I, EcoT14 I |
Nhe I,Spe I,Xba I | CCTAG | - | |||
BmeT110 I | C↓YCGRG | C 3' GGGCT5' |
Ava I, BmeT110 I | CCCGAG | Ava I, BmeT110 I |
C 3' GGGCC5' |
Acc III,Aor13H I,Cfr10 I | CCCGGH | Bcn I, Hap II | ||
Ava I, BmeT110 I | CCCGGG | Ava I, BmeT110 I, Bcn I, Hap II, Sma I | |||
C 3' GAGCT5' |
Ava I, BmeT110 I, Xho I | CTCGAG | Ava I, BmeT110 I, Taq I, Xho I | ||
Sal I | CTCGAC | Taq I | |||
C 3' GAGCC5' |
Ava I, BmeT110 I | CTCGGG | Ava I, BmeT110 I | ||
BmgT120 I | G↓GNCC |
G 3' CCTG5' |
Ava II, BmgT120 I, Cfr13 I,Cpo I,EcoO109 I | GGACC | Ava II, BmgT120 I, Cfr13 I |
G 3' CCGG5' |
BmgT120 I, Cfr13 I, EcoO109 I | GGCCC | BmgT120 I, Cfr13 I, Hae III | ||
G 3' CCCG5' |
BmgT120 I, Cfr13 I | GGGCC | BmgT120 I, Cfr13 I, Hae III | ||
EcoO109 I | GGGCCY | BmgT120 I, Cfr13 I, Hae III Y=C:Apa I, Ban II, Bsp1286 I |
|||
G 3' CCAG5' |
Ava II,Cfr13 I,Cpo I,EcoO109 I | GGTCC | Ava II, Cfr13 I | ||
Bpu1102 I | GC↓TNAGC | GC 3' CGANT5' |
Bpu1102 I | GCTNAGC | Bpu1102 I |
Eco81 I | GCTNAGG | - | |||
BspT104 I | TT↓CGAA | TT 3' AAGC5' |
Acc I,Cla I,Taq I | TTCGA | Taq I |
Hap II | TTCGG | - | |||
Hin1 I | TTCGYC | - | |||
BspT104 I | TTCGAA | BspT104 I, Taq I | |||
Bsp1286 I | GDGCH↓C | GAGCA3' C 5' |
Bsp1286 I | GAGCAC | Bsp1286 I |
GAGCC3' C 5' |
Ban II, Bsp1286 I | GAGCCC | Bsp1286 I | ||
GAGCT3' C 5' |
Ban II, Bsp1286 I, Sac I | GAGCTC | Alu I, Ban II, Bsp1286 I, Sac I | ||
GGGCA3' C 5' |
Bsp1286 I | GGGCAC | Bsp1286 I | ||
GGGCC3' C 5' |
Apa I, Ban II, Bsp1286 I | GGGCCC | Apa I, Ban II, BmgT120 I, Bsp1286 I, Cfr13 I, Hae III | ||
GGGCT3' C 5' | Ban II,Bsp1286 I | GGGCTC | Bsp1286 I | ||
GTGCA3' C 5' |
Bsp1286 I | GTGCAC | ApaL I, Bsp1286 I | ||
EcoT22 I | GTGCAT | - | |||
Pst I,Sse8387 I | GTGCAG | - | |||
GTGCC3' C 5' |
Bsp1286 I | GTGCCC | Bsp1286 I | ||
GTGCT3' C 5' |
Bsp1286 I | GTGCTC | Bsp1286 I | ||
Bsp1407 I | T↓GTACA | T 3' ACATG5' |
Bsp1407 I | TGTACA | Afa I, Bsp1407 I |
BssH II | G↓CGCGC | G 3' CGCGC5' |
BssH II | GCGCGC | Acc II, BssH II, Hha I |
Mlu I | GCGCGT | Acc II, Hha I |
制限酵素 | 認識配列* | 切断後の 末端構造* |
対合末端を生じる 制限酵素 |
対合末端との ライゲーション後の配列* |
ライゲーション後の配列を 認識する制限酵素 |
Bst1107 I | GTA↓TAC | GTA3' CAT5' |
Acc II,Alu I,Bal I,Hae III,Nru I,Pvu II,Stu I | GTAC | Afa I |
Aor51H I | GTAGCT | Alu I | |||
Bst1107 I | GTATAC | Acc I, Bst1107 I | |||
Cfr10 I | R↓CCGGY |
R 3' YGGCC5' |
Acc III,Aor13H I | RCCGGA | Hap II |
Ava I, BmeT110 I | RCCGGG | Bcn I, Hap II | |||
Cfr10 I | RCCGGY | Cfr10 I, Hap II R/Y=G/C:Nae I |
|||
Cfr13 I | G↓GNCC |
G 3' CCTG5' |
Ava II, BmgT120 I, Cfr13 I,Cpo I,EcoO109 I | GGACC | Ava II, BmgT120 I, Cfr13 I |
G 3' CCGG5' |
BmgT120 I, Cfr13 I, EcoO109 I | GGCCC | BmgT120 I, Cfr13 I,Hae III | ||
G 3' CCCG5' |
BmgT120 I, Cfr13 I | GGGCC | BmgT120 I, Cfr13 I, Hae III | ||
EcoO109 I | GGGCCY | BmgT120 I, Cfr13 I, Hae III Y=C:Apa I, Ban II, Bsp1286 I |
|||
G 3' CCAG5' |
Ava II,Cfr13 I,Cpo I,EcoO109 I | GGTCC | Ava II,Cfr13 I | ||
Cla I | AT↓CGAT |
AT 3' TAGC5' |
Acc I,BspT104 I,Taq I | ATCGA | Taq I |
Cla I | ATCGAT | Cla I, Taq I | |||
Hap II | ATCGG | - | |||
Hin1 I | ATCGYC | - | |||
Cpo I | CG↓GWCCG |
CG 3' GCCWG5' |
Ava II, BmgT120 I, Cfr13 I, EcoO109 I | CGGWCC | Ava II, BmgT120 I, Cfr13 I |
Cpo I | CGGWCCG | Ava II, BmgT120 I, Cfr13 I, Cpo I | |||
Dra I | TTT↓AAA | TTT3' AAA5' |
Dra I | TTTAAA | Dra I, RspRS II |
Nru I | TTTCGA | Taq I | |||
Swa I | TTTAAAT | Dra I, RspRS II | |||
Eae I | Y↓GGCCR |
Y 3' RCCGG5' |
Eae I | YGGCCR | Eae I, Hae III Y/R=C/G:Eco52 I Y/R=T/A:Bal I |
Eco52 I,Not I | YGGCCG | Eae I, Hae III Y/C:Eco52 I |
|||
EcoO109 I | RG↓GNCCY |
RG 3' YCCTG5' |
Ava II, BmgT120 I, Cfr13 I, Cpo I | RGGACC | Ava II, BmgT120 I, Cfr13 I |
EcoO109 I | RGGACCY | Ava II, BmgT120 I, Cfr13 I,EcoO109 I | |||
RG 3' YCCGG5' |
BmgT120 I, Cfr13 I | RGGCCC | BmgT120 I, Cfr13 I, Hae III R=G:Apa I, Ban II, Bsp1286 I |
||
EcoO109 I | RGGCCCY | BmgT120 I, Cfr13 I, EcoO109 I, Hae III R=G:Apa I, Ban II, Bsp1286 I |
|||
RG 3' YCCCG5' |
BmgT120 I, Cfr13 I | RGGGCC | BmgT120 I, Cfr13 I, Hae III R=G:Apa I, Ban II, Bsp1286 I |
||
EcoO109 I | RGGGCCY | BmgT120 I, Cfr13 I, EcoO109 I, Hae III Y=C:Apa I, Ban II, Bsp1286 I |
|||
RG 3' YCCAG5' |
Ava II, BmgT120 I, Cfr13 I, Cpo I | RGGTCC | Ava II, BmgT120 I, Cfr13 I | ||
EcoO109 I | RGGTCCY | Ava II, BmgT120 I, Cfr13 I, EcoO109 I | |||
EcoR I | G↓AATTC |
G 3' CTTAA5' |
EcoR I | GAATTC | EcoR I |
Mun I | GAATTG | - | |||
EcoR V | GAT↓ATC | GAT3' CTA5' |
Acc II,Alu I,Bal I,Hae III,Pvu II,Stu I | GATC | Sau3A I |
EcoR V | GATATC | EcoR V | |||
Nru I | GATCGA | Sau3A I, Taq I |
制限酵素 | 認識配列 | 切断後の末端構造* | 対合末端を生じる制限酵素 | 対合末端とのライゲーション後の配列* | ライゲーション後の配列を認識する制限酵素 |
EcoT14 I | C↓CWWGG |
C 3' GGTTC5' |
EcoT14 I | CCAAGG | EcoT14 I |
C 3' GGTAC5' |
EcoT14 I,Nco I | CCATGG | EcoT14 I, Nco I | ||
C 3' GGATC5' |
Bln I, EcoT14 I | CCTAGG | Bln I, EcoT14 I | ||
Nhe I,Spe I,Xba I | CCTAG | - | |||
C 3' GGAAC5' |
EcoT14 I | CCTTGG | EcoT14 I | ||
EcoT22 I | ATGCA↓T |
ATGCA3' T 5' |
Bsp1286 I,Pst I,Sse8387 I | ATGCA | - |
EcoT22 I | ATGCAT | EcoT22 I | |||
Eco52 I | C↓GGCCG |
C 3' GCCGG5' |
Eae I | CGGCCR | Eae I, Hae III R=G:Eco52 I |
Eco52 I,Not I | CGGCCG | Eae I, Eco52 I, Hae III | |||
Eco81 I | CC↓TNAGG |
CC 3' GGANT5' |
Bpu1102 I | CCTNAGC | - |
Eco81 I | CCTNAGG | Eco81 I | |||
Fba I | T↓GATCA |
T 3' ACTAG5' |
BamH I,Bgl II,Mfl I,Sau3A I | TGATC | Sau3A I |
Fba I | TGATCA | Fba I, Sau3A I | |||
Hae II | RGCGC↓Y |
RGCGC3' Y 5' |
Bbe I | RGCGCC | Hae II,Hha I R=G:Bbe I, Hin1 I |
Hae II | RGCGCY | Hae II, Hha I R/Y=A/T:Aor51H I R/Y=G/C:Bbe I, Hin1 I |
|||
Hae III | GG↓CC | GG3' CC5' |
Bal I,Hae III,Stu I | GGCC | Hae III |
Bst1107 I | GGTAC | Afa I | |||
EcoR V | GGATC | Sau3A I | |||
Hap II (Msp I) |
C↓CGG |
C 3' GGC5' |
Acc I | CCGAC | - |
BspT104 I | CCGAA | - | |||
Cla I | CCGAT | - | |||
Hap II | CCGG | Hap II | |||
Hin1 I | CCGYC | - | |||
Taq I | CCGA | - | |||
Hinc II | GTY↓RAC | GTC3' CAG5' |
Bst1107 I | GTCTAC | Acc I |
Hinc II | GTCRAC | R=G:Acc I, Hinc II, Sal I, Taq I | |||
GTT3' CAA5' |
Hinc II | GTTRAC | Hinc II R=A:Hpa I |
||
Hpa I | GTTAAC | Hinc II, Hpa I, RspRS II | |||
Nru I | GTTCGA | Taq I | |||
Hind III | A↓AGCTT |
A 3' TTCGA5' |
Hind III | AAGCTT | Alu I, Hind III |
Hin1 I | GR↓CGYC |
GR 3' CYGC5' |
Acc I,BspT104 I,Cla I,Taq I | GRCGA | - |
Hap II | GRCGG | - | |||
Hin1 I | GRCGYC | Hin1 I R/Y=G/C;Bbe I, Hae II, Hha I |
|||
Hpa I | GTT↓AAC | GTT3' CAA5' |
Hinc II | GTTRAC | Hinc II R=A:Hpa I |
Hpa I | GTTAAC | Hinc II, Hpa I, RspRS II | |||
Nru I | GTTCGA | Taq I | |||
Kpn I | GGTAC↓C |
GGTAC3' C 5' |
Kpn I | GGTACC | Afa I, Kpn I |
Mbo I →Sau3A I |
制限酵素 | 認識配列* | 切断後の 末端構造* |
対合末端を生じる 制限酵素 |
対合末端との ライゲーション後の配列* |
ライゲーション後の配列を 認識する制限酵素 |
Mfl I | R↓GATCY |
R 3' YCTAG5' |
BamH I,Sau3A I | RGATCC | Mfl I, Sau3A I R=G:BamH I |
Bgl II | RGATCT | Mfl I, Sau3A I R=A:Bgl II |
|||
Fba I | RGATCA | Sau3A I R=T:Fba I |
|||
Mfl I | RGATCY | Mfl I, Sau3A I R/Y=A/T:Bgl II R/Y=G/C:BamH I |
|||
Mlu I | A↓CGCGT |
A 3' TGCGC5' |
BssH II | ACGCGC | Acc II, Hha I |
Mlu I | ACGCGT | Acc II, Mlu I | |||
Msp I→Hap II | |||||
Mun I | C↓AATTG |
C 3' GTTAA5' |
EcoR I | CAATTC | - |
Mun I | CAATTG | Mun I | |||
Mva I | CC↓WGG |
CC 3' GGT5' |
Mva I | CCAGG | Mva I |
Tth111 I | CCANGTC | N=G:Mva I | |||
CC 3' GGA5' |
Mva I | CCTGG | Mva I | ||
Tth111 I | CCTNGTC | N=G:Mva I | |||
Nae I | GCC↓GGC |
GCC3' CGG5' |
Nae I | GCCGGC | Cfr10 I, Hap II, Nae I |
Sma I | GCCGGG | Bcn I, Hap II | |||
Nco I | C↓CATGG |
C 3' GGTAC5' |
EcoT14 I,Nco I | CCATGG | EcoT14 I, Nco I |
Nde I | CA↓TATG |
CA 5' GTAT5' |
Nde I | CATATG | Nde I |
PshB I | CATAA | - | |||
RspRS II | CATAG | - | |||
Xsp I | CATAG | - | |||
Nhe I | G↓CTAGC |
G 3' CGATC5' |
Bln I, EcoT14 I, Spe I, Xba I | GCTAG | - |
Nhe I | GCTAGC | Nhe I | |||
Not I | GC↓GGCCGC |
GC 3' CGCCGG5' |
Eae I | GCGGCCR | Hae III R=G:Eae I, Eco52 I |
Eco52 I | GCGGCCG | Eae I, Eco52 I, Hae III | |||
Not I | GCGGCCGC | Eae I, Eco52 I, Hae III, Not I | |||
Nru I | TCG↓CGA | TCG3' AGC5' |
Acc II | TCGCG | Acc II |
Afa I, Dra I, Hpa I, Sca I, Ssp I | TCGA | Taq I | |||
Bst1107 I | TCGTAC | Afa I | |||
EcoR V | TCGATC | Sau3A I, Taq I | |||
Hinc II | TCGRAC | R=A:Taq I | |||
Nru I | TCGCGA | Acc II, Nru I | |||
Nsp V→BspT104 I | |||||
PmaC I | CAC↓GTG | CAC3' GTG5' |
PmaC I | CACGTG | PmaC I |
SnaB I | CACGTA | - | |||
PshB I | AT↓TAAT |
AT 3' TAAT5' |
Nde I | ATTATG | - |
PshB I | ATTAAT | PshB I, RspRS II | |||
RspRS II | ATTAA | RspRS II | |||
Xsp I | ATTAG | - | |||
Psp1406 I | AA↓CGTT |
AA 3' TTGC5' |
Acc I, BspT104 I, Cla I, Taq I | AACGA | - |
Hap II | AACGG | - | |||
Hin1 I | AACGYC | - | |||
Psp1406 I | AACGTT | Psp1406 I | |||
Pst I | CTGCA↓G |
CTGCA3' G 5' |
Bsp1286 I, EcoT22 I | CTGCA | - |
Pst I, Sse8387 I | CTGCAG | Pst I |
制限酵素 | 認識配列* | 切断後の 末端構造* |
対合末端を生じる 制限酵素 |
対合末端との ライゲーション後の配列* |
ライゲーション後の配列を 認識する制限酵素 |
Pvu I | CGAT↓CG | CGAT3' GC 5' |
Pvu I | CGATCG | Pvu I, Sau3A I |
Pvu II | CAG↓CTG | CAG3' GTC5' |
Alu I | CAGCT | Alu I |
Bst1107 I | CAGTAC | Afa I | |||
EcoR V | CAGATC | Sau3A I | |||
Pvu II | CAGCTG | Alu I, Pvu II | |||
RspRS II | T↓TAA | T3' AAT5' |
Nde I | TTAT | - |
RshB I | TTAA | RspRS II | |||
RspRS II | TTAA | RspRS II | |||
Xsp I | TTAG | - | |||
Sac I | GAGCT↓C | GAGCT3' C 5' |
Ban II, Bsp1286 I, Sac I | GAGCTC | Alu I, Ban II, Bsp1286 I, Sac I |
Sac II | CCGC↓GG | CCGC3' GG 5' |
Sac II | CCGCGG | Acc II, Sac II |
Sal I | G↓TCGAC | G 3' CAGCT5' |
Ava I, BmeT110 I, Xho I | GTCGAG | Taq I |
Sal I | GTCGAC | Acc I, Hinc II, Sal I, Taq I | |||
Sau3A I (Mbo I) |
↓GATC | 3' CTAG5' |
BamH I, Bgl II, Fba I, Mfl I, Sau3A I | GATC | Sau3A I |
Sca I | AGT↓ACT | AGT3' TCA5' |
Afa I | AGTAC | Afa I |
Nru I | AGTCGA | Taq I | |||
Sca I | AGTACT | Afa I, Sca I | |||
Sma I | CCC↓GGG | CCC3' GGG5' |
Nae I | CCCGGC | Bcn I, Hap II |
Sma I | CCCGGG | Ava I, BmeT110 I, Bcn I, Hap II, Sma I | |||
Smi I | ATTT↓AAAT |
ATTT3' TAAA5' |
Dra I | ATTTAAA | Dra I |
Nru I | ATTTCGA | Taq I | |||
Swa I | ATTTAAAT | Dra I, Swa I, RspRS II | |||
Spe I | A↓CTAGT |
A 3' TGATC5' |
Bln I, EcoT14 I, Nhe I, Xba I | ACTAG | - |
Spe I | ACTAGT | Spe I | |||
Sse8387 I | CCTGCA↓GG |
CCTGCA3' GG 5' |
Bsp 1286 I, EcoT22 I | CCTGCA | - |
Pst I | CCTGCAG | Pst I | |||
Sse8387 I | CCTGCAGG | Pst I, Sse8387 I | |||
Ssp I | AAT↓ATT | AAT3' TTA5' |
Nru I | AATCGA | Taq I |
Ssp I | AATATT | Ssp I | |||
Stu I | AGG↓CCT | AGG3' TCC5' |
Bal I, Hae III | AGGCC | Hae III |
Bst1107 I | AGGTAC | Afa I | |||
EcoR V | AGGATC | Sau3A I | |||
Stu I | AGGCCT | Hae III, Stu I | |||
Taq I | T↓CGA | T 3' AGC5' |
Acc I, BspT104 I, Cla I, Taq I | TCGA | Taq I |
Hap II | TCGG | - | |||
Hin1 I | TCGYC | - | |||
VpaK11B I→Ava II | |||||
Xba I | T↓CTAGA | T 3' AGATC5' |
Bln I, EcoT14 I, Nhe I, Spe I | TCTAG | - |
Xba I | TCTAGA | Xba I | |||
Xho I | C↓TCGAG | C 3' GAGCT5' |
Ava I, BmeT110 I, Xho I | CTCGAG | Ava I, BmeT110 I, Taq I, Xho I |
Sal I | CTCGAC | Taq I | |||
Xsp I | C↓TAG | C 3' GAT5' |
Nde I | CTATG | - |
PshB I | CTAGT | - | |||
RspRS II | CTAA | - | |||
Xsp I | CTAG | Xsp I |