■アイソシゾマー（isoschizomer）とは
アイソシゾマーは同じ塩基配列を認識する起源の異なる制限酵素です。弊社ウェブカタログやデータシートで制限酵素名のあとに（ ）で表記している酵素はアイソシゾマーです。
認識配列は同じですが、切断位置やメチル化の影響などは異なる場合があります。
切断位置は下記一覧でご確認ください。
弊社で販売している制限酵素のメチル化の影響はこちらをご覧ください。
アイソシゾマーは同じ塩基配列を認識する起源の異なる制限酵素です。弊社ウェブカタログやデータシートで制限酵素名のあとに（ ）で表記している酵素はアイソシゾマーです。
認識配列は同じですが、切断位置やメチル化の影響などは異なる場合があります。
切断位置は下記一覧でご確認ください。
弊社で販売している制限酵素のメチル化の影響はこちらをご覧ください。
| * R | ： | A or G | K | ： | G or T | H | ： | A or C or T | |
| Y | ： | C or T | M | ： | A or C | V | ： | A or C or G | |
| W | ： | A or T | B | ： | C or G or T | N | ： | A or C or G or T | |
| S | ： | G or C | D | ： | A or G or T | ||||
| ● | 認識部位は5'→3'の方向に一本鎖のみ書いており、切断点は矢印（↓）で示した。矢印がないものは、正確な切断点が判明していないことを示す。 | ||||||||
| ● | Eco31 IやFok I等のように認識部位と切断点が離れている酵素は、切断点をカッコ内に示した。 | ||||||||
| 例としてEco31 I GGTCTC（1/5）の切断点を下に示す。 | |||||||||
| 5' GGTCTCN↓ 3' | |||||||||
| 3' CCAGAGN NNNN↑5' | |||||||||
| ● | Nci I は、3'-リン酸になるように切断する。 | ||||||||
| ● | 表中にボールドで示した制限酵素はプロトタイプ（その塩基配列の認識・切断を最初に確認された制限酵素）です。 | ||||||||
| ★ | は、タカラバイオで販売している酵素です。 | ||||||||
※一覧は2004年現在の情報を元に作成したものです。
| 制限酵素 | 認識配列* |
| Aar I | CACCTGC(4/8) |
| ★Aat II Ppu1253 I Ssp5230 I Zra I |
GACGT↓C GACGTC GACGT↓C GAC↓GTC |
| ★Acc I Dsa VI Fbl I Xmi I |
GT↓MKAC GTMKAC GT↓MKAC GT↓MKAC |
| Ace III | CAGCTC(7/11) |
| Aci I | CCGC(-3/-1) |
| Acl I ★Psp1406 I |
AA↓CGTT AA↓CGTT |
| Acy I Aha II Aos II AstW I Asu III Bbi II BsaH I BstAC I Hgi I HgiD I HgiG I HgiH II ★Hin1 I Hin8 I Hsp92 I Msp17 I NlaS II Pam II SspJ II SspM1 II SspM2 II Uba1381 I |
GR↓CGYC GR↓CGYC GR↓CGYC GR↓CGYC GR↓CGYC GR↓CGYC GR↓CGYC GR↓CGYC GR↓CGYC GR↓CGYC GR↓CGYC GR↓CGYC GR↓CGYC GRCGYC GR↓CGYC GR↓CGYC GRCGYC GR↓CGYC GRCGYC GRCGYC GRCGYC GRCGYC |
| ★Afl II Bfr I BsaF I BspT I Bst98 I Cfr92 I Esp4 I MspC I Uba1265 I Uba1266 I Uba1299 I Uba1312 I Uba1313 I Uba1331 I Uba1374 I Uba1420 I Uba1426 I Uba1443 I Vfi I Vha464 I |
C↓TTAAG C↓TTAAG CTTAAG C↓TTAAG C↓TTAAG CTTAAG C↓TTAAG C↓TTAAG CTTAAG CTTAAG CTTAAG CTTAAG CTTAAG CTTAAG CTTAAG CTTAAG CTTAAG CTTAAG CTTAAG C↓TTAAG |
| Afl III | A↓CRYGT |
| Age I AsiA I AsiG I BshT I CspA I PinA I |
A↓CCGGT A↓CCGGT A↓CCGGT A↓CCGGT A↓CCGGT A↓CCGGT |
| Aha III ★Dra I |
TTT↓AAA TTT↓AAA |
| Alf I | GCANNNNNN↓TGC |
| Alo I | (7/12)GAACNNNNNNTCC(12/7) |
| ★Alu I BsaL I Mlt I Otu I OtuN I Oxa I Uba1433 I Uba1441 I |
AG↓CT AGCT AG↓CT AGCT AGCT AGCT AGCT AGCT |
| AlwN I CaiI |
CAGNNN↓CTG CAGNNN↓CTG |
| ★Apa I Bsp120 I EciE I PspOM I Psp30 I Uba1156 I Uba1157 I Uba1165 I Uba1202 I Uba1241 I Uba1368 I |
GGGCC↓C G↓GGCCC GGGCCC G↓GGCCC GGGCCC GGGCCC GGGCCC GGGCCC GGGCCC GGGCCC GGGCCC |
| ApaB I BstAP I |
GCANNNNN↓TGC GCANNNN↓NTGC |
| ★ApaL I Aaq I Alw44 I Ame I Bsp146 I Pfl23 I Pfr12 I Pli I Sno I Uba44 I Uba1203 I Uba1387 I Vne I |
G↓TGCAC GTGCAC G↓TGCAC GTGCAC GTGCAC GTGCAC GTGCAC GTGCAC G↓TGCAC G↓TGCAC GTGCAC GTGCAC G↓TGCAC |
| Apo I Acs I Fsi I XapI |
R↓AATTY R↓AATTY R↓AATTY R↓AATTY |
| Asc I PalA I Sgs I |
GG↓CGCGCC GG↓CGCGCC GG↓CGCGCC |
| AspCN I | GCCGC |
| Asu I AhaB1 I Apu I AspS9 I Avc I Bac36 I Bal228 I BavA II BavB II Bce22 I BmgT120 I BsaS I BshK I BsiZ I BspB II BspF4 I Bsp1894 I Bsu54 I CfrN I Cfr4 I Cfr8 I Cfr13 I Cfr23 I Cfr33 I Cfr45 I Cfr46 I Cfr47 I Cfr52 I Cfr54 I Eco39 I Eco47 II Eco196 II Eco201 I Fmu I Gse I Hin5 II Mja II Msp24 I MthB I MthT I NlaD II NmuE II NmuS I Nsp IV NspL II Nsp7121 I Pde12 I Pph1579 I Pph1773 I Pse I Psp I SauB I Sau2 I Sau5 I Sau13 I Sau14 I Sau17 I Sau96 I Sdy I Uba1099 I Uba1134 I Uba1160 I Uba1164 I Unb I VpaK15 I VpaK25 I |
G↓GNCC GGNCC GGNCC G↓GNCC G↓GNCC G↓GNCC G↓GNCC G↓GNCC G↓GNCC G↓GNCC G↓GNCC GGNCC G↓GNCC G↓GNCC G↓GNCC G↓GNCC G↓GNCC G↓GNCC GGNCC GGNCC GGNCC G↓GNCC GGNCC GGNCC GGNCC GGNCC GGNCC GGNCC GGNCC GGNCC GGNCC GGNCC GGNCC GGNC↓C GGNCC GGNCC GGNCC GGNCC GGNCC GGNCC GGNCC GGNCC GGNCC G↓GNCC GGNCC GGNCC G↓GNCC GGNCC GGNCC GGNCC GGNCC GGNCC GGNCC GGNCC GGNCC GGNCC GGNCC G↓GNCC GGNCC GGNCC GGNCC GGNCC GGNCC ↓GGNCC GGNCC GGNCC |
| Asu II Aca I Acp I Asp10H I Avi I Bim I Bim19 I Bpu14 I BsiC I ★BspT104 I Bsp82 I Bsp119 I Bsp148 I BstB I Cbi I Csp45 I Fsp II Lsp I Mla I Nsp V NspB I NspF I NspJ I Nsp29132 I Osp i Pla II Sfu I Sgr1839 I SspRF I Ssp1 I Ssp48 I Ssp152 I Uba1385 I Uba1452 I |
TT↓CGAA TTCGAA TT↓CGAA TT↓CGAA TTCGAA TT↓CGAA TT↓CGAA TT↓CGAA TT↓CGAA TT↓CGAA TTCGAA TT↓CGAA TTCGAA TT↓CGAA TT↓CGAA TT↓CGAA TT↓CGAA TT↓CGAA TT↓CGAA TT↓CGAA TTCGAA TTCGAA TTCGAA TTCGAA TTCGAA TT↓CGAA TT↓CGAA TTCGAA TT↓CGAA TT↓CGAA TTCGAA TT↓CGAA TTCGAA TTCGAA |
| Ava I Acr I Ama87 I Aqu I AspB I AspC I AspD I Avr I Bco I ★BmeT110 I BsiHKC I BsoB I BspLU4 I BstS I Bst7Q I Eco27k I Eco88 I EspHK29 I Nli I Nli3877 I NmuA I Nsp III NspD I NspE I NspSA I Uba1205 II Uba1436 I Uba1440 I Umi5 I |
C↓YCGRG CYCGRG C↓YCGRG C↓YCGRG CYCGRG CYCGRG CYCGRG CYCGRG C↓YCGRG C↓YCGRG C↓YCGRG C↓YCGRG C↓YCGRG C↓YCGRG CYCGRG C↓YCGRG C↓YCGRG CYCGRG CYCGRG CYCGR↓G CYCGRG C↓YCGRG CYCGRG CYCGRG C↓YCGRG CYCGRG CYCGRG CYCGRG CYCGRG |
| Ava II Afl I AspB II AspC II AspD II Asp697 I Asp745 I BamNx I Bme18 I Bme216 I BspF53 I BspJ105 I Bsp71 I Bsp100 I Bsp128 I Bsp132 I Bsp133 I Bsp1260 I BsrA I Bst4Q I BthA I Bti I Cau I Cli I Clm II Dsa1 V EagM I Eco47 I Erp I Fdi I FspMS I GspA I HgiB I HgiC II HgiE I HgiH III HgiJ I Hsp2 I Kzo49 I Mfo I Mli I MspA I Nli II Nli3877 II NmuA II NspD II NspG I NspH II NspK I Pfl19 I Psp03 I Sfn I Sgh I Sin I SinA I SinB I SinC I SinD I SinE I SinF I SinG I SinH I SinJ I SmuE I Syn I Tru I Uba48 I Uba62 I Uba1131 I |
G↓GWCC G↓GWCC GGWCC GGWCC GGWCC GGWCC G↓GWCC G↓GWCC G↓GWCC G↓GWCC GGWCC GGWCC GGWCC GGWCC GGWCC GGWCC GGWCC GGWCC GGWCC GGWCC G↓GWCC GGWCC G↓GWCC GGWCC GGWCC G↓GWCC GGWCC G↓GWCC G↓GWCC G↓GWCC G↓GWCC GGWCC G↓GWCC G↓GWCC G↓GWCC G↓GWCC G↓GWCC GGWCC G↓GWCC GGWCC GGWCC GGWCC GGWCC GGWCC GGWCC GGWCC GGWCC GGWCC GGWCC GGWCC GGWC↓C GGWCC GGWCC G↓GWCC GGWCC GGWCC GGWCC GGWCC GGWCC GGWCC GGWCC GGWCC GGWCC G↓GWCC GGWCC GGWCC GGWCC GGWCC GGWCC |
| Uba1249 I Uba1272 I Uba1278 I Uba1304 I Uba1314 I Uba1373 I Uba1413 I Uba1438 I VpaK11 I VpaK11A I ★VpaK11B I VpaK65 I |
GGWCC GGWCC GGWCC GGWCC GGWCC GGWCC GGWCC GGWCC GGWCC ↓GGWCC G↓GWCC GGWCC |
| Ava III BfrB I BfrC I ★EcoT22 I Mph1103 I Nsi I PinB I Ppu10 I Sep I SmuC I Uba1353 I Uba1367 I Uba1384 I Zsp2 I |
ATGCAT ATG↓CAT ATGCAT ATGCA↓T ATGCA↓T ATGCA↓T ATGCA↓T A↓TGCAT ATGCA↓T ATGCAT ATGCAT ATGCAT ATGCAT ATGCA↓T |
| Avr II AspA2 I AvrB II ★Bln I Bsp2A I XmaJ I |
C↓CTAGG C↓CTAGG C↓CTAGG C↓CTAGG C↓CTAGG C↓CTAGG |
| Bae I | (10/15)ACNNNNGTAYC(12/7) |
| ★Bal I Mls I MluN I Mlu31 I Msc I Msp20 I |
TGG↓CCA TGG↓CCA TGG↓CCA TGG↓CCA TGG↓CCA TGG↓CCA |
| ★BamH I Aac I Aae I Aca II AccEB I Ain II Ali II Ali12257 I Ali12258 I ApaC I AspT II Atu1 II BamF I BamK I BamN I Bca1259 I Bce751 I Bco10278 I Bna I BsaD I Bsp30 I Bsp46 I Bsp98 I Bsp130 I Bsp131 I Bsp144 I Bst I BstQ I Bst1126 I Bst2464 I Bst2902 I Bst8565 I Bst8566 I Bsu90 I Bsu8646 I Cel I Dds I Gdo I Gin I Gox I Gse III Mle I Mlu23 I NasB I NspSA IV Nsp29132 II Pac1110 I Par177 I Psp56 I Rhs I Rlu4 I Sur I Uba19 I Uba31 I Uba38 I Uba51 I Uba88 I Uba1098 I Uba1163 I Uba1167 I Uba1172 I Uba1173 I Uba1205 I Uba1224 I Uba1242 I Uba1250 I Uba1258 I Uba1297 I Uba1302 I Uba1324 I Uba1325 I Uba1334 I Uba1339 I Uba1346 I Uba1383 I Uba1398 I Uba1402 I Uba1414 I |
G↓GATCC GGATCC GGATCC GGATCC G↓GATCC GGATCC G↓GATCC GGATCC GGATCC GGATCC GGATCC GGATCC GGATCC GGATCC GGATCC GGATCC G↓GATCC GGATCC G↓GATCC GGATCC GGATCC GGATCC GGATCC GGATCC GGATCC GGATCC G↓GATCC GGATCC GGATCC GGATCC GGATCC GGATCC GGATCC GG↓ATCC GGATCC GGATCC GGATCC GGATCC GGATCC GGATCC GGATCC GGATCC G↓GATCC GGATCC G↓GATCC GGATCC GGATCC GGATCC GGATCC GGATCC GGATCC G↓GATCC GGATCC GGATCC GGATCC GGATCC GGATCC GGATCC GGATCC GGATCC GGATCC GGATCC GGATCC GGATCC GGATCC GGATCC GGATCC GGATCC GGATCC GGATCC GGATCC GGATCC GGATCC GGATCC GGATCC GGATCC GGATCC GGATCC |
| Bbv I AlwX I BsaU I BseX I Bsp423 I BsrV I BsrV1 I Bst12 I Bst71 I Lsp1109 I |
GCAGC(8/12) GCAGC(8/12) GCAGC GCAGC(8/12) GCAGC(8/12) GCAGC GCAGC(8/12) GCAGC(8/12) GCAGC(8/12) GCWGC |
| Bbv II Bbr7 I Bbs I Bbv16 II Bco102 II Bpi I BpuA I BsaVI Bsc91 I Bsp VI Bst V2I |
GAAGAC(2/6) GAAGAC(7/11) GAAGAC(2/6) GAAGAC(2/6) GAAGAC GAAGAC(2/6) GAAGAC(2/6) GAAGAC GAAGAC(2/6) GAAGAC GAAGAC(2/6) |
| BbvC I | CC↓TCAGC |
| Bcc I | CCATC |
| Bcef I BceA I |
ACGGC(12/13) ACGGC(12/14) |
| Bce83 I BpuE I |
CTTGAG(16/14) CTTGAG(16/14) |
| Bcg I | (10/12)GCANNNNNNTCG(12/10) |
| BciV I Bfu I |
GTATCC(6/5) GTATCC(6/5) |
| Bcl I AtuC I Bco102 I BsiQ I BspX II BstG I BstK I Bst77 I Cpe I Cth I ★Fba I Ksp22 I Pov I Sse I Sst IV Uba1282 I Uba1283 I Uba1431 I Uba1447 I Umi7 I |
T↓GATCA TGATCA TGATCA T↓GATCA T↓GATCA TGATCA TGATCA TGATCA TGATCA TGATCA T↓GATCA T↓GATCA T↓GATCA TGATCA TGATCA TGATCA TGATCA TGATCA TGATCA TGATCA |
| Bet I Bca77 I BsaW I Bst1473 I |
W↓CCGGW W↓CCGGW W↓CCGGW WCCGGW |
| Bfi I BmrI |
ACTGGG(5/4) ACTGGG(5/4) |
| ★Bgl I Van I |
GCCNNNN↓NGGC GCCNNNNNGGC |
| ★Bgl II Ncr I NspMAC I Pae2k I Pae18k I |
A↓GATCT A↓GATCT A↓GATCT A↓GATCT A↓GATCT |
| Bin I AclW I Alw I BspP I BsrW I Bth II Ral8 I |
GGATC(4/5) GGATC(4/5) GGATC(4/5) GGATC(4/5) GGATC GGATC GGATC |
| Bmg I | GKGCCC |
| Bpl I | (8/13)GAGNNNNNCTC(13/8) |
| Bpu10 I | CCTNAGC(-5/-2) |
| BsaA I BstBA I MspY I Ppu6 I Ppu11 I Ppu21 I |
YAC↓GTR YAC↓GTR YAC↓GTR YACGTR YACGTR YACGTR |
| BsaB I Bco63 I BseJ I Bse8 I Bsh1365 I BsiB I BsrBR I Mam I |
GATNN↓NNATC GATNNNNATC GATNN↓NNATC GATNN↓NNATC GATNN↓NNATC GATNN↓NNATC GATNN↓NNATC GATNN↓NNATC |
| BsaX I | (9/12)ACNNNNNCTCC(10/7) |
| Bsb I | CAACAC |
| BscG I BpuJ I |
CCCGT CCCGT |
| BseM II BspCN I |
CTSAG(10/8) CTCAG(9/7) |
| BseP I BscE I BsoP I BsrH I ★BssH II CfrJ5 I Eco143 I Eco152 I Esp7 I Esp8 I Kpn30 I Pau I Uba69 I |
G↓CGCGC GCGCGC GCGCGC GCGCGC G↓CGCGC GCGCGC GCGCGC GCGCGC GCGCGC GCGCGC GCGCGC G↓CGCGC GCGCGC |
| BseR I | GAGGAG(10/8) |
| BseS I Bme1580 I BseS I |
GKGCM↓C GKGCM↓C GKGCM↓C |
| BseY I | C↓CCAGC |
| Bsg I | GTGCAG(16/14) |
| Bsi I BssS I Bst2B I |
CTCGTG(-5/-1) CTCGTG(-5/-1) CTCGTG(-5/-1) |
| BsiY I Bsc4 I Bsc107 I BseL I Bse23 I Bsl I |
CCNNNNN↓NNGG CCNNNN↓NNNGG CCNNNNNNNGG CCNNNNN↓NNGG CCNNNNNNNGG CCNNNNN↓NNGG |
| Bsm I Asp26H I Asp27H I Asp35H I Asp36H I Asp40H I Asp50H I BsaM I BscC I Mva1269 I Pct I Uba1382 I Uba1415 I |
GAATGC(1/-1) GAATGC(1/-1) GAATGC(1/-1) GAATGC(1/-1) GAATGC(1/-1) GAATGC(1/-1) GAATGC(1/-1) GAATGC(1/-1) GAATGC(1/-1) GAATGC(1/-1) GAATGC(1/-1) GAATGC GAATGC |
| BsmA I Alw26 I BsoMA I |
GTCTC(1/5) GTCTC(1/5) GTCTC(1/5) |
| BspG I | CTGGAC |
| BspH I Pag I Rca I Rhc I RspX I |
T↓CATGA T↓CATGA T↓CATGA TCATGA TCATGA |
| BspLU11 I Pci I Psc I |
A↓CATGT A↓CATGT A↓CATGT |
| BspM I Acc36 I BfuA I |
ACCTGC(4/8) ACCTGC(4/8) ACCTGC(4/8) |
| BspM II ★Acc III ★Aor13H I Bbf7411 I BbvA III Bla7920 I BlfI BseA I BsiM I BspE I Bsp13 I Bsu22 I Bsu23 I CauB3 I Cfr57 I EspHK26 I Kpn2 I Mro I PinB II Pta I Uba1136 I Uba1279 I Uba1375 I Uba1425 I |
T↓CCGGA T↓CCGGA T↓CCGGA TCCGGA T↓CCGGA TCCGGA T↓CCGGA T↓CCGGA T↓CCGGA T↓CCGGA T↓CCGGA TCCGGA T↓CCGGA T↓CCGGA TCCGGA TCCGGA T↓CCGGA T↓CCGGA T↓CCGGA T↓CCGGA TCCGGA TCCGGA TCCGGA TCCGGA |
| BspNC I | CCAGA |
| Bsp24 I | (8/13)GACNNNNNNTGG(12/7) |
| ★Bsp1407 I Aau I BsrG I BstAU I Bst170 I SspB I |
T↓GTACA T↓GTACA T↓GTACA T↓GTACA TGTACA T↓GTACA |
| 制限酵素 | 認識配列* |
| Bsr I BscH I BseN I Bse1 I BsrS I Bst11 I Tsp1 I |
ACTGG(1/-1) ACTGG ACTGG(1/-1) ACTGG(1/-1) ACTGG(1/-1) ACTGG(1/-1) ACTGG(1/-1) |
| BsrB I AccBS I Mbi I |
GAGCGG(-3/-3) GAGCGG(-3/-3) CCGCTC(-3/-3) |
| BsrD I Bse3D I BseM I |
GCAATG(2/0) GCAATG(2/0) GCAATG(2/0) |
| BstE II Acr II AspA I Bse59 I Bse64 I BstD I ★BstP I Bst31 I Cfr7 I Cfr19 I Eca I Eci125 I ★EcoO65 I EcoO128 I Eco91 I Kox I NspSA II PspE I SciA I Uba1291 I |
G↓GTNACC G↓GTNACC G↓GTNACC GGTNACC GGTNACC GGTNACC G↓GTNACC GGTNACC GGTNACC GGTNACC G↓GTNACC G↓GTNACC G↓GTNACC G↓GTNACC G↓GTNACC G↓GTNACC G↓GTNACC G↓GTNACC GGTNACC GGTNACC |
| ★BstX I BscJ I BssG I BstT I |
CCANNNNN↓NTGG CCANNNNNNTGG CCANNNNNNTGG CCANNNNNNTGG |
| BtgZ I | GCGATG(10/14) |
| Btr I BmgB I |
CACGTC(-3/-3) CACGTC(-3/-3) |
| Bts I | GCAGTG(2/0) |
| Cac8 I BstC8 I |
GCN↓NGC GCN↓NGC |
| Cau II Aha I Ase II Asp1 I AsuC2 I ★Bcn I BspF105 I BspJ67 I Bsp7 I Bsp8 I Bsp55 I EciD I EcoH I Eco121 I Eco179 I Eco190 I HgiS21 I HgiS22 I Hin3 I Kpn49k II Nci I Pae181 I Rsh II Ssp2 I Tmu1 I Uba41 I Uba42 I Uba1280 I Uba1318 I Uba1347 I Uba1370 I Uba1372 I Uba1376 I Uba1378 I Uba1389 I Uba1401 I Uba1423 I Uba1424 I |
CC↓SGG CC↓SGG CCSGG CCSGG CC↓SGG CC↓SGG CCSGG CCSGG CCSGG CCSGG CCSGG CCSGG ↓CCSGG CCSGG CCSGG CCSGG CCSGG CC↓SGG CCSGG ↓CCSGG CC↓SGG CCSGG CCSGG CCSGG CCSGG CCSGG CCSGG CCSGG CCSGG CCSGG CCSGG CCSGG CCSGG CCSGG CCSGG CCSGG CCSGG CCSGG |
| Cdi I | CATCG |
| Cfr I Cfr14 I Cfr38 I Cfr39 I Cfr40 I Cfr55 I Cfr59 I ★Eae I EciB I EcoHA I Eco90 I Eco164 I EspHK16 I EspHK24 I KspHK15 I Uba36 I Uba1188 I Uba1327 I |
Y↓GGCCR YGGCCR YGGCCR YGGCCR YGGCCR YGGCCR YGGCCR Y↓GGCCR YGGCCR YGGCCR YGGCCR YGGCCR YGGCCR YGGCCR YGGCCR YGGCCR YGGCCR YGGCCR |
| ★Cfr10 I Bco118 I Bse118 I Bsp21 I BsrF I BssA I |
R↓CCGGY R↓CCGGY R↓CCGGY RCCGGY R↓CCGGY R↓CCGGY |
| Cje I | (8/14)CCANNNNNNGT(15/9) |
| CjeN II | GAGNNNNNGT |
| CjeP I | (7/13)CCANNNNNNNTC(14/8) |
| ★Cla I Aag I Apu16 I Asp707 I Ban III BavC I BbvA II BciB I Bci29 I Bcm I Bdi I BfrA I BliR I Bli41 I Bli86 I Bsa29 I Bsc I BseC I BsiX I BspD I BspJ II BspX I Bsp2 I Bsp4 I Bsp84 I Bsp106 I Bsp125 I Bsp126 I Bsp127 I Bsp145 I BsrC I BstLV I Bst28 I Bsu15 I BsuTU I Btu I Csp4 I Lpl I Pga I Rme21 I Spm I Uba22 I Uba24 I Uba30 I Uba34 I Uba43 I Uba1096 I Uba1100 I Uba1133 I Uba1137 I Uba1138 I Uba1144 I Uba1145 I Uba1161 I Uba1168 I Uba1195 I Uba1196 I Uba1197 I Uba1198 I Uba1199 I Uba1200 I Uba1233 I Uba1238 I Uba1246 I Uba1257 I Uba1275 I Uba1286 I Uba1295 I Uba1315 I Uba1342 I Uba1366 II Uba1379 I Uba1380 I Uba1394 I Uba1412 I Uba1416 I Uba1427 I Uba1430 I Uba1451 I Uba1453 I Zho I |
AT↓CGAT AT↓CGAT ATCGAT ATCGAT AT↓CGAT ATCGAT AT↓CGAT AT↓CGAT AT↓CGAT AT↓CGAT AT↓CGAT ATCGAT AT↓CGAT AT↓CGAT AT↓CGAT AT↓CGAT AT↓CGAT AT↓CGAT AT↓CGAT AT↓CGAT AT↓CGAT AT↓CGAT ATCGAT ATCGAT ATCGAT AT↓CGAT ATCGAT ATCGAT ATCGAT ATCGAT ATCGAT ATCGAT AT↓CGAT AT↓CGAT AT↓CGAT ATCGAT ATCGAT AT↓CGAT AT↓CGAT AT↓CGAT AT↓CGAT ATCGAT ATCGAT ATCGAT ATCGAT ATCGAT ATCGAT ATCGAT ATCGAT ATCGAT ATCGAT ATCGAT ATCGAT ATCGAT ATCGAT ATCGAT ATCGAT ATCGAT ATCGAT ATCGAT ATCGAT ATCGAT ATCGAT ATCGAT ATCGAT ATCGAT ATCGAT ATCGAT ATCGAT ATCGAT ATCGAT ATCGAT ATCGAT ATCGAT ATCGAT ATCGAT ATCGAT ATCGAT ATCGAT ATCGAT AT↓CGAT |
| CspC I | (11/13)CAANNNNNGTGG(12/10) |
| CctM I | AAGGAG(20/18) |
| CviJ I CviK I CviL I CviM I CviN I CviO I CviT I |
RG↓CY RGCY RGCY RGCY RGCY RGCY RG↓CY |
| CviR I HpyCH4 V |
TG↓CA TG↓CA |
| ★Dde I BstDE I HpyF3 I |
C↓TNAG C↓TNAG C↓TNAG |
| ★Dpn I Cfu I Mal I Mph1103 II Nan II NgoDX IV NmuD I NmuE I NsuD I |
G6mA↓TC G6mA↓TC G6mA↓TC G6mATC G6mATC G6mATC G6mATC G6mATC |
| Dra II ★EcoO109 I Pss I Uba1326 I VneA I |
RG↓GNCCY RG↓GNCCY RGGNC↓CY RGGNCCY RGGNCCY |
| Dra III Ade I |
CACNNN↓GTG CACNNN↓GTG |
| Drd I Aas I DseD I |
GACNNNN↓NNGTC GACNNNN↓NNGTC GACNNNN↓NNGTC |
| Drd II | GAACCA |
| Dsa I BstDS I Btg I |
C↓CRYGG C↓CRYGG C↓CRYGG |
| Eam1105 I Ahd I AspE I EclHK I NruG I Uba1190 I Uba1191 I |
GACNNN↓NNGTC GACNNN↓NNGTC GACNNN↓NNGTC GACNNN↓NNGTC GACNNN↓NNGTC GACNNN↓NNGTC GACNNN↓NNGTC |
| Eci I BpuC I |
GGCGGA(11/9) GGCGGA |
| EcoN I BstEN I BstW I Uba1268 I Uba1289 I Uba1290 I Uba1308 I Uba1309 I Uba1310 I Xag I |
CCTNN↓NNNAGG CCTNN↓NNNAGG CCTNNNNNAGG CCTNNNNNAGG CCTNNNNNAGG CCTNNNNNAGG CCTNNNNNAGG CCTNNNNNAGG CCTNNNNNAGG CCTNN↓NNNAGG |
| ★EcoR I Eco82 I Eco159 I Eco228 I Eco237 I Eco252 I Fun II Hal I Kpn49k I Rsr I Sso I Uba58 I Van91 II |
G↓AATTC GAATTC GAATTC GAATTC GAATTC GAATTC G↓AATTC G↓AATTC G↓AATTC G↓AATTC G↓AATTC GAATTC GAATTC |
| EcoR II Acc38 I Aeu I Ajn I Aor I ApaOR I Apy I Asp2H I Atu II AtuB I Atu1 I ★BciT130 I BinS I Bpt I BsaN I BseB I Bse16 I Bse17 I Bse24 I BshG I Bsi VI BsiL I BsoG I BspN I Bsp56 I Bsp103 I Bsp317 I BstG II BstM6 I BstN I BstO I Bst1 I Bst2 I Bst2U I Bst7Q II Bst38 I Bst100 I BthD I BthE I Cdi27 I CfrS37 I Cfr5 I |
↓CCWGG CCWGG CCWGG ↓CCWGG CC↓WGG CC↓WGG CC↓WGG CCWGG CC↓WGG CCWGG CCWGG CC↓WGG CCWGG CC↓WGG CCWGG CC↓WGG CC↓WGG CC↓WGG CC↓WGG CC↓WGG CCWGG CC↓WGG CCWGG CC↓WGG CCWGG CCWGG CCWGG CCWGG CC↓WGG CC↓WGG CC↓WGG CCWGG CC↓WGG CC↓WGG CCWGG CC↓WGG CC↓WGG CC↓WGG CC↓WGG CCWGG CCWGG CCWGG |
| 制限酵素 | 認識配列* |
| Cfr11 I Cfr20 I Cfr22 I Cfr24 I Cfr25 I Cfr27 I Cfr28 I Cfr29 I Cfr30 I Cfr31 I Cfr35 I Cfr58 I Cth II EagK I Eca II Ecl II EclS39 I Ecl66 I Ecl136 I Ecl137 II Eco38 I Eco40 I Eco41 I Eco60 I Eco61 I Eco67 I Eco70 I Eco71 I Eco128 I Eco136 I Eco165 I Eco170 I Eco193 I Eco206 I Eco207 I Eco254 I Eco256 I Ese6 II EspHK7 I EspHK22 I EspHK30 I Esp2 I Esp24 I Fsp1604 I Hhd I Kox165 I Kpn10 I Kpn13 I Kpn14 I Kpn16 I KspHK12 I KspHK14 I Mlu2300 I Mph I Mva I PspG I Psp6 I Sau16 I Scg2 I Sfl2a I Sfl2b I Sgr II Sgr20 I Sle I Sni I Ssl I SspA I Sth117 I TaqX I TspA I Uba11 I Uba13 I Uba20 I Uba81 I Uba82 I Uba1114 I Uba1118 I Uba1120 I Uba1121 I Uba1125 I Uba1171 I Uba1181 I Uba1185 I Uba1189 I Uba1193 I Uba1218 I Uba1243 I Uba1410 I Uba1428 I Zan I |
CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CC↓WGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CC↓WGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CC↓WGG ↓CCWGG ↓CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CC↓WGG CC↓WGG CCWGG CC↓WGG CC↓WGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CCWGG CC↓WGG |
| ★EcoR V BshL I BsoA I Bsp16 I BstR I Ceq I Eco32 I Eco178 I Hja I Nan I NflA I NsiC I Pac1110 II Pfl16 I Uba1400 I |
GAT↓ATC GATATC GATATC GATATC GATATC GAT↓ATC GAT↓ATC GATATC GATATC GATATC GATATC GATATC GATATC GATATC GATATC |
| Eco31 I Bli49 I Bli5508 I Bsa I Bso31 I BspTN I Cfr56 I EcoA4 I Eco42 I Eco51 I Eco95 I Eco97 I Eco101 I Eco120 I Eco127 I Eco129 I Eco155 I Eco156 I Eco157 I Eco162 I Eco185 I Eco191 I Eco203 I Eco204 I Eco205 I Eco217 I Eco225 I Eco233 I Eco239 I Eco240 I Eco241 I Eco246 I Eco247 I Eco263 I Ppa I Rle69 I Sau12 I Uba65 I Uba84 I Uba1316 I Uba1343 I |
GGTCTC(1/5) GGTCTC GGTCTC GGTCTC(1/5) GGTCTC(1/5) GGTCTC(1/5) GGTCTC GGTCTC(1/5) GGTCTC GGTCTC GGTCTC GGTCTC GGTCTC GGTCTC GGTCTC GGTCTC GGTCTC GGTCTC GGTCTC GGTCTC GGTCTC GGTCTC GGTCTC GGTCTC GGTCTC GGTCTC GGTCTC GGTCTC GGTCTC GGTCTC GGTCTC GGTCTC GGTCTC GGTCTC GGTCTC GGTCTC GGTCTC GGTCTC GGTCTC GGTCTC GGTCTC |
| Eco47 III Afe I Ait I ★Aor51H I Fun I |
AGC↓GCT AGC↓GCT AGCGCT AGC↓GCT AGC↓GCT |
| Eco57 I Acu I Bsp6 II Eco112 I Eco125 I Fsf I |
CTGAAG(16/14) CTGAAG(16/14) CTGAAG CTGAAG CTGAAG CTGAAG |
| Eco57M I | CTGRAG(16/14) |
| Esp I Blp I ★Bpu 1102 I Bsp 1720 I Cel II Uba 1221 I Uba 1222 I Uba 1284 I Uba 1320 I |
GC↓TNAGC GC↓TNAGC GC↓TNAGC GC↓TNAGC GC↓TNAGC GCTNAGC GCTNAGC GCTNAGC GCTNAGC |
| Esp3 I BsmB I Esp16 I Esp23 I |
CGTCTC(1/5) CGTCTC(1/5) GAGACG GAGACG |
| Fal I | (8/13)AAGNNNNNCTT(13/8) |
| Fau I Smu I |
CCCGC(4/6) CCCGC(4/6) |
| Fin I BpuS I BslF I BsmF I |
GGGAC GGGAC(10/14) (11/15)GGGAC(10/14) GGGAC(10/14) |
| FnuD II ★Acc II BceR I Bep I Bpu95 I Bsh1236 I BspJ76 I Bsp50 I Bsp70 I BstFN I BstU I BsuE II Bsu1192 II Bsu1193 I Bsu1532 I Bsu6633 I Btk I CpaA I Cpa1150 I FspM I Hin1056 I MvaA I Mvn I PflA I SceA I Sel I Tha I Tma I Uba1321 I Uba1404 I Uba1405 I Uba1446 I |
CG↓CG CG↓CG CGCG CGCG CG↓CG CG↓CG CGCG CGCG CGCG CG↓CG CG↓CG CGCG CGCG CGCG CG↓CG CGCG CG↓CG CGCG CGCG CGCG CGCG CGCG CG↓CG CGCG CGCG ↓CGCG CG↓CG CGCG CGCG CGCG CGCG CGCG |
| Fnu4H I Bis I BsoF I Bsp6 I BssF I BssX I BthC I Cac824 I Fbr I Fsp4H I Isp I Ita I Sat I Uur960 I |
GC↓NGC GC↓NGC GC↓NGC GC↓NGC GCNGC GCNGC GCNG↓C GCNGC GC↓NGC GC↓NGC GC↓NGC GC↓NGC GC↓NGC GC↓NGC |
| ★Fok I BseG I BstF5 I HinGU II Sts I |
GGATG(9/13) GGATG(2/0) GGATG(2/0) GGATG GGATG(10/14) |
| Fse I | GGCCGG↓CC |
| FspA I | RTGC↓GCAY |
| Gdi II | YGGCCG(-5/-1) |
| Gsu I Bco35 I Bpm I BspJ74 I Bsp22 I Bsp28 I Uba1437 I Uba1444 I |
CTGGAG(16/14) CTGGAG CTGGAG(16/14) CTGGAG CTGGAG CTGGAG CTGGAG CTGGAG |
| Hae I | WGG↓CCW |
| ★Hae II AccB2 I Bme142 I BsmH I Bsp143 II BstH2 I Btu34 II HinH I Lpn I Ngo I |
RGCGC↓Y RGCGC↓Y RGC↓GCY RGCGCY RGCGC↓Y RGCGC↓Y RGCGCY RGCGCY RGC↓GCY RGCGCY |
| ★Hae III Aca IV AspT III Asp742 I AvrB I Bal475 I Bal3006 I Bce71 I Bco33 I Bfi458 I Bim19 II Bli I Blu II BsaR I Bse I BseQ I Bsh I BshA I BshB I BshC I BshD I BshE I BshF I BsiA I BsiD I BsiH I BspBR I BspK I BspR I Bsp71 I Bsp137 I Bsp211 I Bsp226 I Bsp1261 I BssC I BstC I BstJ I BsuR I Bsu1076 I Bsu1114 I Bte I Clm I Clt I Csp2 I Dsa II FinS I FnuD I |
GG↓CC GGCC GGCC GGCC GGCC GGCC GGCC GGCC GGCC GGCC GG↓CC GGCC GGCC GGCC GGCC GG↓CC GG↓CC GGCC GGCC GGCC GGCC GGCC GGCC GGCC GGCC GGCC GG↓CC GG↓CC GG↓CC GGCC GGCC GG↓CC GGCC GGCC GGCC GGCC GGCC GG↓CC GGCC GGCC GGCC GGCC GG↓CC GGCC GG↓CC GGCC GG↓CC |
| 制限酵素 | 認識配列* |
| Hhg I MfoA I Mni I NgoA II NgoP II NgoS II Nla I Och I Pai I Pal I Pde133 I PflK I Pla I Ple214 I Ppu I Psp29 I Sbv I Sfa I Spl III Sua I Sul I TspZN I TteA I Ttn I Uba9 I Uba54 I Uba61 I Uba1097 I Uba1140 I Uba1146 I Uba1147 I Uba1150 I Uba1152 I Uba1153 I Uba1155 I Uba1169 I Uba1174 I Uba1175 I Uba1176 I Uba1178 I Uba1179 I Uba1207 I Uba1208 I Uba1209 I Uba1210 I Uba1214 I Uba1223 I Uba1228 I Uba1230 I Uba1231 I Uba1235 I Uba1288 I Uba1292 I Uba1293 I Uba1319 I Uba1322 I Uba1336 I Uba1377 I Uba1388 I Uba1392 I Uba1395 I Uba1408 I Uba1418 I Uba1422 I Uba1429 I Uba1449 I Uba1450 I Van91 III Vha I Vha1168 I Vni I |
GGCC GG↓CC GGCC GGCC GGCC GGCC GGCC GGCC GGCC GG↓CC GG↓CC GG↓CC GG↓CC GGCC GGCC GGCC GG↓CC GG↓CC GGCC GG↓CC GGCC GGCC GGCC GGCC GGCC GGCC GGCC GGCC GGCC GGCC GGCC GGCC GGCC GGCC GGCC GGCC GGCC GGCC GGCC GGCC GGCC GGCC GGCC GGCC GGCC GGCC GGCC GGCC GGCC GGCC GGCC GGCC GGCC GGCC GGCC GGCC GGCC GGCC GGCC GGCC GGCC GGCC GGCC GGCC GGCC GGCC GGCC GGCC GGCC GGCC GGCC |
| Hae IV | (7/13)GAYNNNNNRTC(14/9) |
| Hga I | GACGC(5/10) |
| HgiA I Alw21 I AspH I Bbv12 I BsaG I Bsh45 I BsiHKA I BsmP I Pph3215 I |
GWGCW↓C GWGCW↓C GWGCW↓C GWGCW↓C GWGCWC GWGCW↓C GWGCW↓C GWGCWC GWGCW↓C |
| HgiC I AccB1 I Ban I BbvB I BshN I ★BspT107 I Eco50 I Eco64 I Eco168 I Eco169 I Eco171 I Eco173 I Eco195 I Esp1 I Esp6 I Esp9 I Esp10 I Esp11 I Esp12 I Esp13 I Esp14 I Esp15 I Esp21 I Esp22 I Esp25 I HgiH I MspB4 I Pph14 I SspM1 III Uba1127 I Vsp2246 I |
G↓GYRCC G↓GYRCC G↓GYRCC G↓GYRCC G↓GYRCC G↓GYRCC GGYRCC G↓GYRCC GGYRCC GGYRCC GGYRCC GGYRCC GGYRCC GGYRCC GGYRCC GGYRCC GGYRCC GGYRCC GGYRCC GGYRCC GGYRCC GGYRCC GGYRCC GGYRCC GGYRCC G↓GYRCC G↓GYRCC GGYRCC GGYRCC GGYRCC GGYRCC |
| HgiE II | ACCNNNNNNGGT |
| HgiJ II ★Ban II Bsp117 I Bsp519 I Bsu1854 I Bvu I Cfr48 I EcoT38 I EcoT88 I EcoT93 I EcoT95 I Eco24 I Eco25 I Eco26 I Eco35 I Eco68 I Eco113 I Eco180 I Eco211 I Eco215 I Eco216 I Eco232 I Eco249 I Eco262 I Ese4 I FriO I Kox II PaeH I Ppu20 I Psp31 I SacN I Uba39 I Uba57 I Uba1124 I Uba1159 I Uba1206 I Uba1263 I Uba1264 I Uba1307 I Uba1329 I Uba1330 I Uba1357 I Uba1363 I Uba1409 I Uba1421 I |
GRGCY↓C GRGCY↓C GRGCYC GRGCY↓C GRGCY↓C GRGCY↓C GRGCYC GRGCYC GRGCYC GRGCYC GRGCYC GRGCY↓C GRGCYC GRGCYC GRGCYC GRGCYC GRGCYC GRGCYC GRGCYC GRGCY↓C GRGCYC GRGCYC GRGCYC GRGCYC GRGCYC GRGCY↓C GRGCY↓C GRGCY↓C GRGCYC GRGCYC GRGCY↓C GRGCYC GRGCYC GRGCYC GRGCYC GRGCYC GRGCYC GRGCYC GRGCYC GRGCYC GRGCYC GRGCYC GRGCYC GRGCYC GRGCYC |
| ★Hha I AspLE I Bca I BstHH I Cfo I Csp1470 I FnuD III HinGU I HinP1 I HinS1 I HinS2 I Hin6 I Hin7 I HspA I Mnn IV SciNI |
GCG↓C GCG↓C GCGC GCG↓C GCG↓C GCGC GCG↓C GCGC G↓CGC GCGC GCGC G↓CGC GCGC G↓CGC GCGC G↓CGC |
| Hind II Chu II ★Hinc II HinJC I Hin1160 II Hin1161 II Mnn I |
GTY↓RAC GTYRAC GTY↓RAC GTY↓RAC GTYRAC GTYRAC GTYRAC |
| ★Hind III Asp52 I Asp3065 I Bbr I Bpe I BseH I BstF I Bst170 II Cfr32 I Chu I Eco VIII Eco65 I Eco98 I Eco188 I Eco231 I Hinb III Hinf II HinJC II Hin5 III Hin173 I Hin1076 III Hsu I Mki I Ssb I Uba83 I Uba1164 II Uba1219 I Uba1435 I |
A↓AGCTT AAGCTT AAGCTT AAGCTT AAGCTT AAGCTT A↓AGCTT AAGCTT AAGCTT AAGCTT A↓AGCTT AAGCTT AAGCTT AAGCTT AAGCTT AAGCTT AAGCTT AAGCTT AAGCTT AAGCTT AAGCTT A↓AGCTT AAGCTT A↓AGCTT AAGCTT AAGCTT AAGCTT AAGCTT |
| ★Hinf I CviB I CviC I CviD I CviE I CviF I CviG I FnuA I Hha II HpyA IV Nca I NsiH I Nov II |
G↓ANTC G↓ANTC GANTC GANTC GANTC GANTC GANTC G↓ANTC G↓ANTC GANTC GANTC GANTC GANTC |
| Hin4 I | (8/13)GABNNNNNVTC(13/8) |
| Hin4 II HpyA V |
CCTTC CCTTC(6/5) |
| ★Hpa I BsaK I Bse II BstHP I KspA I Ssr I Uba1408 II |
GTT↓AAC GTTAAC GTTAAC GTT↓AAC GTT↓AAC GTTAAC GTTAAC |
| Hpa II Asp748 I Bco27 I BsaZ I BshM I BsiS I Bsp5 I Bsp47 I Bsp48 I Bsp116 I Bst40 I BsuF I Bsu1192 I Fin II ★Hap II Hin2 I Hin5 I Mni II Mno I ★Msp I Pde137 I Pme35 I Sec II SfaGU I Sth134 I Uba1128 I Uba1141 I Uba1267 I Uba1338 I Uba1355 I Uba1439 I |
C↓CGG CCGG C↓CGG CCGG CCGG C↓CGG CCGG CCGG CCGG CCGG C↓CGG CCGG CCGG CCGG C↓CGG C↓CGG CCGG CCGG C↓CGG C↓CGG C↓CGG CCGG CCGG CCGG C↓CGG CCGG CCGG CCGG CCGG CCGG CCGG |
| Hph I AsuHP I NgoB VIII SspD5 I |
GGTGA(8/7) GGTGA(8/7) GGTGA GGTGA(8/8) |
| Hpy99 I | CGWCG↓ |
| Hpy178 III Hpy188 III |
TC↓NNGA TC↓NNGA |
| Hpy188 I | TCN↓GA |
| ★Kpn I Acc65 I AhaB8 I Asp718 I BspJ106 I Eco149 I Esp19 I KpnK14 I Nmi I Sau10 I Sth I SthA I SthB I SthC I SthD I SthE I SthF I SthG I SthH I SthJ I SthK I SthL I SthM I SthN I Uba76 I |
GGTAC↓C G↓GTACC G↓GTACC G↓GTACC GGTACC GGTACC GGTACC GGTACC GGTACC GGTACC G↓GTACC GGTACC GGTACC GGTACC GGTACC GGTACC GGTACC GGTACC GGTACC GGTACC GGTACC GGTACC GGTACC GGTACC GGTACC |
| 制限酵素 | 認識配列* |
| Uba85 I Uba86 I Uba87 I Uba1201 I |
GGTACC GGTACC GGTACC GGTACC |
| Ksp632 I Bco5 I Bco116 I BseZ I BsrE I Bst6 I Bst158 I Bsu6 I Eam1104 I Ear I Uba1192 I Uba1276 I |
CTCTTC(1/4) CTCTTC(1/4) CTCTTC(1/4) CTCTTC(1/4) CTCTTC CTCTTC(1/4) CTCTTC CTCTTC(1/4) CTCTTC(1/4) CTCTTC(1/4) CTCTTC CTCTTC |
| Mae I Bfa I FspB I Mja I MthF I MthZ I Rma I ★Xsp I |
C↓TAG C↓TAG C↓TAG CTAG CTAG C↓TAG C↓TAG C↓TAG |
| Mae II HpyCH4 IV Tai I Tsc I |
A↓CGT A↓CGT ACGT↓ ACGT↓ |
| Mae III | ↓GTNAC |
| ★Mbo I AspMD I Bce243 I Bme12 I BsaP I BscF I Bsp I BspA I BspF I BspJ I BspJ64 I BspKT6 I Bsp9 I Bsp18 I Bsp49 I Bsp51 I Bsp52 I Bsp54 I Bsp57 I Bsp58 I Bsp59 I Bsp60 I Bsp61 I Bsp64 I Bsp65 I Bsp66 I Bsp67 I Bsp72 I Bsp74 I Bsp76 I Bsp91 I Bsp105 I Bsp122 I Bsp135 I Bsp136 I Bsp138 I Bsp143 I Bsp147 I Bsp2095 I BsrM I BsrP II BssG II BstE III BstKT I BstMB I BstX II Btc I Btk II Btu33 I Btu34 I Btu36 I Btu37 I Btu39 I Btu41 I Cac I Ccy I Cha I Cin1467 I Cpa I Cpf I Csp5 I Cte1179 I Cte1180 I Cty I CviA I CviB II CviH I Dpn II FnuA II FnuC I FnuE I Hac I Kzo9 I LlaA I Lsp1109 II Meu I MkrA I Mme II Mno III Mos I MspB I Msp67 II Mth I MthA I Mth1047 I Nde II Nfl I NflA II NflB I Nla II NlaD I NmeC I Nph I NsiA I NspA I Nsu I Pei9403 I Pfa I Pph288 I Rlu1 I SalA I SalH I ★Sau3A I SauC I SauD I SauE I SauF I SauG I SauM I Sau15 I Sau6782 I SinM I Tru II Uba4 I Uba59 I Uba1101 I Uba1177 I Uba1182 I Uba1183 I Uba1204 I Uba1259 I Uba1317 I Uba1323 I Uba1336 I Vha44 I |
↓GATC ↓GATC ↓GATC GATC GATC GATC GATC ↓GATC ↓GATC ↓GATC GATC GAT↓C GATC GATC GATC GATC GATC GATC GATC GATC GATC GATC GATC GATC GATC GATC ↓GATC GATC GATC GATC GATC ↓GATC GATC GATC GATC GATC ↓GATC GATC ↓GATC GATC GATC GATC GATC GAT↓C ↓GATC GATC GATC ↓GATC GATC GATC GATC GATC GATC GATC ↓GATC ↓GATC GATC↓ GATC GATC ↓GATC GATC GATC GATC GATC ↓GATC GATC GATC ↓GATC GATC ↓GATC ↓GATC ↓GATC ↓GATC ↓GATC GATC GATC GATC GATC GATC GATC GATC GATC GATC GATC GATC ↓GATC GATC GATC GATC ↓GATC GATC ↓GATC ↓GATC GATC GATC GATC GATC GATC GATC GATC GATC GATC ↓GATC GATC GATC GATC GATC GATC GATC GATC GATC GATC GATC GATC GATC GATC GATC GATC GATC GATC GATC GATC GATC GATC GATC |
| ★Mbo II Ncu I Tce I |
GAAGA(8/7) GAAGA GAAGA |
| Mcr I BsaO I Bsh1285 I BsiE I BstMC I Uba1303 I |
CGRY↓CG CGRY↓CG CGRY↓CG CGRY↓CG CGRY↓CG CGRYCG |
| Mfe I ★Mun I |
C↓AATTG C↓AATTG |
| Mja IV Hpy8 I |
GTNNAC GTNNAC |
| ★Mlu I Ape I Uba6 I |
A↓CGCGT ACGCGT ACGCGT |
| Mme I | TCCRAC(20/18) |
| Mnl I | CCTC(7/6) |
| Mse I ★RspRS II SaqA I Tru1 I Tru9 I |
T↓TAA T↓TAA T↓TAA T↓TAA T↓TAA |
| Msl I SmiM I |
CAYNN↓NNRTG CAYNN↓NNRTG |
| Mst I Aca III Acc16 I Aos I Avi II Bco6 I BsaT I Clc II Cli II Fdi II Fsp I GspA II Nsb I NspH III NspL I NspM I Pam I |
TGC↓GCA TGCGCA TGC↓GCA TGC↓GCA TGC↓GCA TGCGCA TGCGCA TGCGCA TGCGCA TGC↓GCA TGC↓GCA TGCGCA TGC↓GCA TGCGCA TGCGCA TGCGCA TGC↓GCA |
| Mwo I Bce1247 I BspW I HpyF10 VI |
GCNNNNN↓NNGC GCNNNNNNNGC GCNNNNN↓NNGC GCNNNNNN↓NGC |
| ★Nae I Afa24R I Ame II AniM I ApeA I Apr I Eco56 I Esp5 I MauA I Mis I Mlu9273 II MroN I NasW I Nba I Nbr I NgoA IV NgoM IV Nmu I NmuF I NspW I NtaS II Pgl I Psp61 I Rlu I SacA I SalC I Sao I SauA I SauBMK I SauHP I SauLP I SauN I SauS I Ska I Slu1777 I Smo40529 I SspC I Uba1122 I |
GCC↓GGC GCCGGC GCCGGC GCCGGC GCCGGC GCCGGC G↓CCGGC GCCGGC GCCGGC GCCGGC GCCGGC G↓CCGGC GCCGGC GCCGGC GCCGGC G↓CCGGC G↓CCGGC GCCGGC GCCGGC GCCGGC GCCGGC GCCGGC GCCGGC GCCGGC GCCGGC GCCGGC GCCGGC GCCGGC GCC↓GGC GCC↓GGC GCC↓GGC GCC↓GGC GCC↓GGC GCCGGC GCC↓GGC GCCGGC GCC↓GGC GCCGGC |
| Nar I Bbe I BbeA I BinS II Eco78 I Ege I Ehe I Kas I McaA I Mch I Mly113 I Msa I Nam I Nda I Nun II Pmn I Sfo I SseA I |
GG↓CGCC GGCGC↓C GGCGCC GGCGCC GGC↓GCC GGC↓GCC GGC↓GCC G↓GCGCC GGCGCC GG↓CGCC GG↓CGCC GGCGCC GGCGCC GG↓CGCC GG↓CGCC GGC↓GCC GGCGCC GG↓CGCC |
| ★Nco I Ate I Bsp19 I NspSA III |
C↓CATGG CCATGG C↓CATGG CCATGG |
| ★Nde I FauND I |
CA↓TATG CA↓TATG |
| ★Nhe I Ace II Bmt I PstNH I |
G↓CTAGC GCTAG↓C GCTAG↓C G↓CTAGC |
| Nla III CviA II Fat I Hin1 II Hin8 II HpyCH4 I Hsp92 II |
CATG↓ C↓ATG ↓CATG CATG CATG CAT↓G CATG↓ |
| Nla IV AspN I Bcr I BsaE I BscB I BspL I Bsp29 I Bss I PspN4 I Rlu3 I Uba1305 I Uba1445 I |
GGN↓NCC GGN↓NCC GGNNCC GGNNCC GGN↓NCC GGN↓NCC GGNNCC GGNNCC GGN↓NCC GGNNCC GGNNCC GGNNCC |
| ★Not I CciN I |
GC↓GGCCGC GC↓GGCCGC |
| ★Nru I Ama I Bsp68 I MluB2 I Mlu9273 I PspD I SalD I Sbo13 I Sna3286 I |
TCG↓CGA TCGCGA TCG↓CGA TCG↓CGA TCGCGA TCGCGA TCGCGA TCG↓CGA TCGCGA |
| 制限酵素 | 認識配列* |
| Spo I Uba1117 I Uba1386 I |
TCG↓CGA TCGCGA TCGCGA |
| Nsp I BstNS I Lsp1270 I NspH I Xce I |
RCATG↓Y RCATG↓Y RCATGY RCATG↓Y RCATG↓Y |
| NspB II MspA1 I |
CMG↓CKG CMG↓CKG |
| Oli I Ale I |
CACNN↓NNGTG CACNN↓NNGTG |
| Pac I | TTAAT↓TAA |
| Pas I | CC↓CWGGG |
| PflM I AccB7 I Acp II Asp10H II Esp1396 I ★Van91 I |
CCANNNN↓NTGG CCANNNN↓NTGG CCANNNN↓NTGG CCANNNN↓NTGG CCANNNN↓NTGG CCANNNN↓NTGG |
| Pfl1108 I | TCGTAG |
| Pfo I | T↓CCNGGA |
| Ple I BsmE I Mly I PpsI SchI |
GAGTC(4/5) GAGTC GAGTC（5/5） GAGTC（4/5） GAGTC（5/5） |
| ★PmaC I BbrP I BcoA I Bsp87 I Eco72 I Pgl34 I Pml I PspB I Psp38 I |
CAC↓GTG CAC↓GTG CAC↓GTG CACGTG CAC↓GTG CACGTG CAC↓GTG CACGTG CACGTG |
| Pme I MssI |
GTTT↓AAAC GTTT↓AAAC |
| Ppi I | (7/12)GAACNNNNNCTC(13/8) |
| PpuM I Mlu1106 I Pfl27 I PspPP I Psp5 II |
RG↓GWCCY RGGWCCY RG↓GWCCY RG↓GWCCY RG↓GWCCY |
| PshA I Box I BstPA I |
GACNN↓NNGTC GACNN↓NNGTC GACNN↓NNGTC |
| PsiI | TTA↓TAA |
| PspX I | VC↓TCGAGB |
| Psr I | (7/12)GAACNNNNNNTAC(12/7) |
| ★Pst I Ain I AliAJ I Ali2882 I Api I AspT I Asp36 I Asp708 I Asp713 I Bbi I Bce170 I BmeB I BsaN II BsaQ I BscD I BspB I Bsp17 I Bsp43 I Bsp63 I Bsp78 I Bsp81 I Bsp93 I Bsp107 I Bsp108 I Bsp268 I BsuB I Cau III Cfl I CfrA4 I Cfu II Clc I Cst I EaeP I Ecl77 I Ecl133 I Ecl593 I Eco48 I Eco49 I Eco83 I Eco133 I Eco141 I Eco161 I Eco167 I Eco260 I Eco261 I Esp5 II Esp141 I Gse II Hal II Kpn12 I Mau I MhaA I Miz I Mkr I Nas I Ngb I Noc I PaeP I Pae8 I Pae9 I Pae14 I Pae15 I Pae22 I Pae24 I Pae25 I Pae26 I Pae39 I Pae40 I Pae41 I Pfl37 I Pma I Pma44 I Pmy I Pph2059 I PspS I Psp28 I Psp46 I SalP I Sal13 I ScoA I Sfl I Ska II SprL I UbaHKB I Uba46 I Uba71 I Uba72 I Uba1112 I Uba1115 I Uba1116 I Uba1119 I Uba1123 I Uba1149 I Uba1184 I Uba1186 I Uba1211 I Uba1212 I Uba1213 I Uba1215 I Uba1216 I Uba1225 I Uba1232 I Uba1256 I Uba1262 I Uba1287 I Uba1294 II Uba1296 I Uba1328 I Uba1337 I Uba1399 I Uba1411 I Uba1417 I Xma II Xor I Xph I Yen I YenA I YenB I YenC I YenD I YenE I |
CTGCA↓G CTGCAG CTGCA↓G CTGCAG CTGCA↓G CTGCAG CTGCAG CTGCAG CTGCA↓G CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCA↓G CTGCAG CTGCAG CTGCA↓G CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCA↓G CTGCA↓G CTGCA↓G CTGCAG CTGCA↓G CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCA↓G CTGCAG CTGCAG CTGCA↓G CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCA↓G CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCA↓G CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCA↓G CTGCAG CTGCAG CTGCA↓G CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG CTGCA↓G CTGCAG CTGCAG CTGCAG CTGCAG CTGCAG |
| ★Pvu I Afa16R I Afa22M I Bma I BmaA I BmaB I BmaC I BmaD I BspC I Cas2 I Cfr51 I Drd III EclJ I ErhB9 I Kpl79 I Nbl I Ple19 I Psu161 I Pvu84 I Rsh I Rsp I SmaA III SplA III Uba1129 I Uba1139 I Xgl3216 I Xgl3217 I Xgl3218 I Xgl3219 I Xgl3220 I Xml I XmlA I Xni I Xor II |
CGAT↓CG CGA↓TCG CGA↓TCG CGA↓TCG CGATCG CGATCG CGATCG CGATCG CGAT↓CG CGATCG CGATCG CGATCG CGATCG CGAT↓CG CGATCG CGAT↓CG CGAT↓CG CGAT↓CG CGATCG CGAT↓CG CGATCG CGATCG CGATCG CGATCG CGATCG CGATCG CGATCG CGATCG CGATCG CGATCG CGATCG CGATCG CGATCG CGAT↓CG |
| ★Pvu II Bav I BavA I BavB I BspO4 I Cfr6 I Dma I Gsp I Mzi I NmeR I Psp3 I Psp5 I PvuHKu I Pvu84 II Sba I SciA II SmaA IV Sol3335 I SplA IV Uba1227 I Uba1245 I |
CAG↓CTG CAG↓CTG CAG↓CTG CAG↓CTG CAG↓CTG CAG↓CTG CAG↓CTG CAGCTG CAGCTG CAGCTG CAGCTG CAGCTG CAGCTG CAG↓CTG CAGCTG CAGCTG CAGCTG CAGCTG CAGCTG CAGCTG CAGCTG |
| RleA I | CCCACA(12/9) |
| Rsa I ★Afa I Asp 16H I Asp 17H I Asp 18H I Asp 29H I Csp 6 I CviQ I CviR II |
GT↓AC GT↓AC GTAC GTAC GTAC GTAC G↓TAC G↓TAC G↓TAC |
| Rsr II ★Cpo I Csp I Rsr2 I |
CG↓GWCCG CG↓GWCCG CG↓GWCCG CG↓GWCCG |
| ★Sac I Ecl136 II Ecl137 II EcoICR I Mxa I NasS I Pfl18 I Psp124B I Sco I Sst I |
GAGCT↓C GAG↓CTC GAGCTC GAG↓CTC GAG↓CTC GAGCTC GAGCTC GAGCT↓C GAGCTC GAGCT↓C |
| ★Sac II Aos III Asp32H I Bac I Bac465 I Bsp12 I Cfr37 I Cfr41 I Cfr42 I Cfr43 I Cfr45 II Csc I Cte1 I DrdA I DrdB I DrdC I DrdE I DrdF I Dsp1 I Eae46 I Ecc I Ecl1 I Ecl28 I Ecl37 I Eco29k I Eco55 I Eco92 I Eco96 I Eco99 I Eco100 I Eco104 I Eco134 I Eco135 I Eco151 I Eco158 I Eco182 I Eco196 I Eco208 I Ese3 I Ese6 I Fsc I Gal I Gce I GceGL I Kpn19 I Kpn378 I |
CCGC↓GG CCGCGG CCGCGG CCGCGG CCGCGG CCGCGG CCGCGG CCGCGG CCGC↓GG CCGCGG CCGCGG CCGC↓GG CCGCGG CCGCGG CCGCGG CCGCGG CCGCGG CCGCGG CCGCGG CCGC↓GG CCGCGG CCGCGG CCGCGG CCGCGG CCGC↓GG CCGCGG CCGCGG CCGCGG CCGCGG CCGCGG CCGCGG CCGCGG CCGCGG CCGCGG CCGCGG CCGCGG CCGCGG CCGCGG CCGCGG CCGCGG CCGCGG CCGC↓GG CCGC↓GG CCGC↓GG CCGC↓GG CCGCGG |
| 制限酵素 | 認識配列* |
| Ksp I Mra I NgoA III NgoD I NgoP III NlaD III NlaS I PaeA I PaeQ I Pae7 I Pae17 I Pae36 I Pae42 I Pae43 I Pae44 I Plf1108 II Saa I Sab I Sak I Sbo I Sfr I Sfr303 I Sfr382 I SgrB I Shy I Spu I Sse II Ssp1725 I Sst II Sta I Tgl I Tto I UbaHKA I Uba66 I Uba77 I Uba90 I Uba1093 I Uba1095 I Uba1111 I Uba1113 I Uba1126 I Uba1187 I Uba1229 I Uba1234 I Uba1244 I Uba1306 I Uba1364 I Uba1369 I |
CCGC↓GG CCGCGG CCGC↓GG CCGCGG CCGC↓GG CCGCGG CCGCGG CCGC↓GG CCGCGG CCGCGG CCGCGG CCGCGG CCGCGG CCGCGG CCGCGG CCGCGG CCGCGG CCGCGG CCGCGG CCGCGG CCGCGG CCGC↓GG CCGCGG CCGC↓GG CCGCGG CCGC↓GG CCGCGG CCGCGG CCGC↓GG CCGCGG CCGCGG CCGCGG CCGCGG CCGCGG CCGCGG CCGCGG CCGCGG CCGCGG CCGCGG CCGCGG CCGCGG CCGCGG CCGCGG CCGCGG CCGCGG CCGCGG CCGCGG CCGCGG |
| ★Sal I Acs1371 I Acs1372 I Acs1373 I Acs1421 I Acs1422 I HgiC III HgiD II Koy I Nop I Psp32 I Psp33 I Psp89 I RflF I Rhe I Rhp I Rrh I Rrh4273 I Rro I Rtr I Uba89 I Xam I Xci I |
G↓TCGAC GTCGAC GTCGAC GTCGAC GTCGAC GTCGAC G↓TCGAC G↓TCGAC GTCGAC G↓TCGAC GTCGAC GTCGAC GTCGAC GTCGAC GTCGAC GTCGAC GTCGAC GTCGAC GTCGAC G↓TCGAC GTCGAC GTCGAC G↓TCGAC |
| SanD I Sse1825 I |
GG↓GWCCC GG↓GWCCC |
| Sap I BspQ I VpaK32 I |
GCTCTTC(1/4) GCTCTTC GCTCTTC(1/4) |
| Sau I Aoc I Axy I BliHK I Bse21 I Bst29 I Bst30 I Bsu36 I Cvn I EciC I Eco76 I ★Eco81 I Eco115 I Eco118 I Lmu60 I Mst II OxaN I SauH I Sec III SshA I Uba1184 I Uba1294 I Uba1332 I Uba1333 I |
CC↓TNAGG CC↓TNAGG CC↓TNAGG CC↓TNAGG CC↓TNAGG CCTNAGG CCTNAGG CC↓TNAGG CC↓TNAGG CCTNAGG CCTNAGG CC↓TNAGG CCTNAGG CCTNAGG CC↓TNAGG CC↓TNAGG CC↓TNAGG CCTNAGG CCTNAGG CC↓TNAGG CCTNAGG CCTNAGG CCTNAGG CCTNAGG |
| ★Sca I Acc113 I Afl IV Asp763 I BshH I BsoS I BstM I Dpa I Eco255 I Pin I RflF II Uba1094 I Uba1158 I |
AGT↓ACT AGT↓ACT AGTACT AGTACT AGTACT AGTACT AGTACT AGT↓ACT AGT↓ACT AGTACT AGT↓ACT AGTACT AGTACT |
| ScrF I Bme1390 I BsaC I Bso I Bsp53 I Bsp73 I BssK I Dsa V Eco43 I Eco51 II Eco80 I Eco85 I Eco93 I Eco153 I Eco200 I MspR9 I Msp67 I Nla X Sso II Uba17 I Uba1391 I |
CC↓NGG CC↓NGG CCNGG CCNGG CCNGG CCNGG ↓CCNGG ↓CCNGG CCNGG CCNGG CCNGG CCNGG CCNGG CCNGG CCNGG CC↓NGG CC↓NGG CCNGG ↓CCNGG CCNGG CCNGG |
| Sdu I Aoc II Bka1125 I Bmy I BsoC I BspLS2 I ★Bsp1286 I Nsp II Nsp7524 II Uba1362 I |
GDGCH↓C GDGCH↓C GDGCHC GDGCH↓C GDGCHC GDGCH↓C GDGCH↓C GDGCH↓C GDGCH↓C GDGCHC |
| Sec I BsaJ I BseD I BssEC I Uba1442 I |
C↓CNNGG C↓CNNGG C↓CNNGG C↓CNNGG CCNNGG |
| SexA I Mab I |
A↓CCWGGT A↓CCWGGT |
| SfaN I BscA I BsmN I Lwe I |
GCATC(5/9) GCATC(4/6) GCATC GCATC(5/9) |
| Sfe I Bco 163 I BdiS I Bfm I Bpc I BstSF I LlaB I Sfc I |
C↓TRYAG CTRYAG C↓TRYAG C↓TRYAG C↓TRYAG C↓TRYAG C↓TRYAG C↓TRYAG |
| ★Sfi I Sdi I |
GGCCNNNN↓NGGCC GGCCNNNN↓NGGCC |
| Sgf I AsiS I Rga I |
GCGAT↓CGC GCGAT↓CGC GCGAT↓CGC |
| SgrA I | CR↓CCGGYG |
| Sim I BtsP I Sai I |
GGGTC(-3/0) GGGTC GGGTC |
| ★Sma I Ahy I CfrJ4 I Cfr9 I EaeA I EclR I PaeB I PspA I PspAL I Uba1220 I Uba1393 I UthS I Xcy I Xma I XmaC I |
CCC↓GGG C↓CCGGG CCC↓GGG C↓CCGGG C↓CCGGG CCCGGG CCC↓GGG C↓CCGGG CCC↓GGG CCCGGG CCCGGG CC↓CGGG C↓CCGGG C↓CCGGG C↓CCGGG |
| Sml I Smo I |
C↓TYRAG C↓TYRAG |
| Sna I BspM90 I BssNA I BstBS I BstZ17 I ★Bst1107 I Xca I |
GTATAC GTA↓TAC GTA↓TAC GTA↓TAC GTATAC GTA↓TAC GTA↓TAC |
| ★SnaB I BstSN I EciA I Eco105 I Eco158 II SspJ I SspM1 I SspM2 I Uba1240 I |
TAC↓GTA TAC↓GTA TACGTA TAC↓GTA TACGTA TACGTA TACGTA TACGTA TACGTA |
| ★Spe I AclN I Ahl I Bcu I |
A↓CTAGT A↓CTAGT A↓CTAGT A↓CTAGT |
| ★Sph I Asp5H I Bbu I Bsp121 I Pae I PaeC I SpaX I Uba1162 I Uba1226 I |
GCATG↓C GCATGC GCATG↓C GCATGC GCATG↓C GCATGC GCATGC GCATGC GCATGC |
| Spl I BpuB5 I BsiW I Pfl23 II PfuN I PpuA I PspL I SmaA I SplA I SspK I Sun I |
C↓GTACG C↓GTACG C↓GTACG C↓GTACG CGTACG C↓GTACG C↓GTACG CGTACG CGTACG CGTACG C↓GTACG |
| Srf I | GCCC↓GGGC |
| Sse232 I Mre I |
CG↓CCGGCG CG↓CCGGCG |
| ★Sse8387 I Sbf I Sda I |
CCTGCA↓GG CCTGCA↓GG CCTGCA↓GG |
| Sse8647 I | AG↓GWCCT |
| ★Ssp I | AAT↓ATT |
| Sth132 I | CCCG(4/8) |
| ★Stu I Aat I Asp78 I Chy I Eco147 I Gdi I GobA I NtaS I Pce I Pme55 I Ppu13 I Sar I SseB I Ssv I Ste I Uba40 I Uba1170 I Uba1180 I Uba1217 I Uba1239 I Uba1371 I Uba1403 I Uba1419 I |
AGG↓CCT AGG↓CCT AGGCCT AGGCCT AGG↓CCT AGG↓CCT AGGCCT AGGCCT AGG↓CCT AGG↓CCT AGGCCT AGG↓CCT AGG↓CCT AGGCCT AGGCCT AGGCCT AGGCCT AGGCCT AGGCCT AGGCCT AGGCCT AGGCCT AGGCCT |
| Sty I BsmS I BssT1 I Bst224 I ★EcoT14 I EcoT104 I Eco130 I Eco208 II Erh I ErhB9 II SblA I SblB I SblC I Uba1311 I |
C↓CWWGG CCWWGG C↓CWWGG CCWWGG C↓CWWGG CCWWGG C↓CWWGG CCWWGG C↓CWWGG C↓CWWGG CCWWGG CCWWGG CCWWGG CCWWGG |
| Swa I ★Smi I |
ATTT↓AAAT ATTT↓AAAT |
| ★Taq I CviS III EsaBC3 I Tfl I TthHB8 I |
T↓CGA TCGA TC↓GA TCGA T↓CGA |
| Taq II | GACCGA(11/9) CACCCA(11/9) |
| Tat I | WGTACW |
| 制限酵素 | 認識配列* |
| Tau I BspU I |
GCSG↓C GCSG↓C |
| Tfi I Pfe I |
G↓AWTC GAWTC |
| Tse I | G↓CWGC |
| Tso I | TARCCA |
| TspDT I | ATGAA(11/9) |
| TspE I MluC I Sse9 I TasI Tsp509 I |
↓AATT AATT ↓AATT ↓AATT ↓AATT |
| TspGW I | ACGGA(11/9) |
| TspR I | CASTGNN↓ |
| Tsp4C I Bst4C I HpyCH4 III TaaI |
ACN↓GT ACN↓GT ACN↓GT ACN↓GT |
| Tsp45 I NmuC I |
↓GTSAC ↓GTSAC |
| Tth111 I Asp I Ats I Fsu I Nta I PflF I PsyI SmaA II Spl II SplA II Tel I Tsp I Tte I Ttr I |
GACN↓NNGTC GACN↓NNGTC GACN↓NNGTC GACNNNGTC GACNNNGTC GACNNNGTC GACN↓NNGTC GACNNNGTC GACNNNGTC GACNNNGTC GACN↓NNGTC GACNNNGTC GACNNNGTC GACNNNGTC |
| Tth111 II | CAARCA(11/9) |
| UbaD I | GAACNNNNNNTCC |
| UbaE I | CACCTGC |
| UbaF2 I | GAAAYNNNNNRTG |
| UbaF3 I | CACNNNNNNTCC |
| UbaF4 I | GAANNNNNNNTTGG |
| UbaF5 I | CTGATG |
| UbaF6 I | GCGAC |
| UbaG I | CACNNNNGTG |
| UbaH I | GCANNNNNNTGC |
| UbaP I | CGAACG |
| UbaT I | TGANNNNNNTCA |
| Vsp I Ase I Asn I PshB I Srl17D I |
AT↓TAAT AT↓TAAT AT↓TAAT AT↓TAAT ATTAAT |
| ★Xba I BspLU11 II BsrX I Msp23 I |
T↓CTAGA TCTAGA TCTAGA TCTAGA |
| Xcm I | CCANNNNN↓NNNNTGG |
| ★Xho I Abr I Asp15 I Asp47 I Asp703 I Bad I Bbf I Bbi III Blu I Bsp92 I Bsp129 I Bsp139 I Bsp140 I Bsp141 I Bsp142 I BssH I BstH I BstL I BstV I BsuM I Bth I Ccr I Cja I Dde II DraD I Eae2 I Mav I Mca I Mec I Mha I MlaA I Mpu I Mrh I MscA I Msi I Msp23 II Oco I PaeR7 I Pan I PflN I PflW I Pfl67 I Psp4 I Sal1974 I Sau3239 I Sbi68 I Sca1827 I Sci I SciB I Sci1831 I Scu I Sex I Sfr274 I Sfu1762 I Sga I Sgo I Sgr1841 I Shy1766 I Sla I Slu I Sol10179 I Spa I Sph1719 I Ssp4 I StaA I Sve194 I Tli I Uba1130 I Uba1148 I Uba1154 I Uba1166 I Uba1237 I Uba1248 I Uba1271 I Uba1298 I Uba1335 I Uba1397 I Uba1448 I Xpa I |
C↓TCGAG C↓TCGAG CTCGAG CTCGAG CTCGAG CTCGAG CTCGAG CTCGAG C↓TCGAG CTCGAG CTCGAG CTCGAG CTCGAG CTCGAG CTCGAG CTCGAG CTCGAG CTCGAG CTCGAG CTCGAG CTCGAG C↓TCGAG CTCGAG CTCGAG CTCGAG CTCGAG C↓TCGAG CTCGAG CTCGAG CTCGAG CT↓CGAG CTCGAG CT↓CGAG CTCGAG CTCGAG CTCGAG CTCGAG C↓TCGAG C↓TCGAG CTCGAG CTCGAG CTCGAG CTCGAG CTCGAG C↓TCGAG C↓TCGAG CTCGAG CTC↓GAG CTCGAG CTCGAG CTCGAG CTCGAG C↓TCGAG CTCGAG CTCGAG CTCGAG CTCGAG CTCGAG C↓TCGAG CTCGAG C↓TCGAG CTCGAG CTCGAG CTCGAG CTCGAG CTCGAG C↓TCGAG CTCGAG CTCGAG CTCGAG CTCGAG CTCGAG CTCGAG CTCGAG CTCGAG CTCGAG CTCGAG CTCGAG C↓TCGAG |
| Xho II Ait II AitA I Asp1H I Asp6H I Asp8H I Asp14H I Asp17 I Asp21H I Asp22 I BstX2 I BstY I Dsa III ★Mfl I Psu I Tru201 I Uba1432 I |
R↓GATCY RGATCY RGATCY RGATCY RGATCY RGATCY RGATCY RGATCY RGATCY RGATCY R↓GATCY R↓GATCY R↓GATCY R↓GATCY R↓GATCY R↓GATCY RGATCY |
| Xma III Aaa I BseX3 I BsoD I BstZ I Eag I EclX I ★Eco52 I |
C↓GGCCG C↓GGCCG C↓GGCCG CGGCCG C↓GGCCG C↓GGCCG C↓GGCCG C↓GGCCG |
| Xmn I Asp700 I BbvA I MroX I Pdm I Syn II |
GAANN↓NNTTC GAANN↓NNTTC GAANN↓NNTTC GAANN↓NNTTC GAANN↓NNTTC GAANNNNTTC |
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