保存 緩衝液 |
Tris-HCl (mM) |
KPO4 (mM) |
pH (25℃) |
KCl (mM) |
NaCl (mM) |
MgCl2 (mM) |
DTT (mM) |
EDTA (mM) |
Triton X-100 (%) |
BSA (%) |
Glycerol (%) |
1 | 10 | - | 7.5 | - | 100 | - | 1 | 0.1 | - | 0.01 | 50 |
2 | - | 10 | 7.4 | - | 200 | - | 1 | 1 | - | 0.02 | 50 |
3 | 10 | - | 7.5 | 100 | - | - | 1 | 0.1 | - | 0.01 | 50 |
4 | 10 | - | 7.5 | 100 | - | - | 1 | 0.1 | - | 0.02 | 50 |
5 | 10 | - | 7.5 | 50 | - | - | 1 | 0.1 | - | 0.01 | 50 |
6 | 10 | - | 7.5 | 200 | - | - | 1 | 0.1 | - | 0.01 | 50 |
7 | 10 | - | 7.5 | 200 | - | - | 1 | 0.1 | 0.15 | 0.02 | 50 |
8 | 10 | - | 7.5 | 400 | - | - | 1 | 0.1 | - | 0.01 | 50 |
9 | 10 | - | 7.5 | 400 | - | - | 1 | 0.1 | 0.15 | 0.01 | 50 |
10 | 10 | - | 7.5 | - | 50 | - | 1 | 0.1 | - | 0.01 | 50 |
11 | 10 | - | 8.0 | 100 | - | - | 1 | 0.1 | 0.15 | 0.01 | 50 |
12 | 10 | - | 8.0 | 100 | - | - | 1 | 0.1 | - | 0.02 | 50 |
13 | 10 | - | 8.0 | 200 | - | - | 1 | 0.1 | - | 0.01 | 50 |
14 | 10 | - | 7.5 | 100 | - | - | 1 | 0.1 | 0.15 | 0.01 | 50 |
15 | 10 | - | 7.5 | 100 | - | - | 1 | 1 | - | 0.01 | 50 |
16 | 10 | - | 7.4 | 100 | - | - | 1 | 1 | - | 0.02 | 50 |
17 | 10 | - | 7.5 | 100 | - | 10 | 1 | 0.1 | - | 0.01 | 50 |
18 | 10 | - | 7.5 | - | 100 | 10 | 1 | 0.1 | 0.15 | 0.01 | 50 |
19 | 10 | - | 7.5 | 300 | - | CaCl2 5 | 1 | 0.1 | 0.15 | 0.02 | 50 |
20 | 10 | - | 7.5 | 300 | - | - | 1 | 0.1 | - | 0.05 | 50 |
21 | 10 | - | 7.5 | 400 | - | - | 1 | 0.1 | - | 0.02 | 50 |
22 | 10 | - | 7.5 | 100 | - | MnCl2 1 | 1 | 0.1 | 0.15 | 0.01 | 50 |
23 | 10 | - | 7.5 | - | 100 | - | 1 | 1 | - | 0.02 | 50 |
保存緩衝液 | 酵素名 | ||||
1 | Smi I | ||||
2 | Bcn I | Cfr10 I | Hin1 I | Msp I | |
3 | Acc III | Bln I | Nco I | ||
4 | Afa I | Aor13H I | Cpo I | Mbo II | Nde I |
PshA I | PshB I | Stu I | |||
5 | Sca I | Sma I | Ssp I | Xba I | |
6 | Bal I | EcoT14 I | |||
7 | Aor51H I | BmgT120 I | Tth111 I | ||
8 | Hinc II | Hind III | Mlu I | Sal I | VpaK11B I |
9 | BamH I | BspT104 I | BstX I | EcoO109 I | Hae III |
10 | Afl II | Sac II |
|||
11 | Pvu I | ||||
12 | Eco52 I | Nru I | Dde I |
||
13 | Bgl I | ||||
14 | Acc I | Acc II | Alu I | Apa I | ApaL I |
Ban II | BmeT110 I | BspT107 I | Bsp1286 I | BssH II | |
BstP I | Cla I | Dra I | Eae I | EcoO65 I | |
EcoR I | EcoR V | Fba I | Fok I | Hap II | |
Hha I | Hinf I | Hpa I | Kpn I | Mbo I | |
Mfl I | Nae I | Nhe I | Not I | PmaC I | |
Pvu II | Sac I | Sau3A I | SnaB I | Spe I | |
Sph I | Sse8387 I | Xho I | Xsp I | ||
15 | Hae II | ||||
16 | Bpu1102 I | Bsp1407 I | Bst1107 I | Eam1105 I | Eco81 I |
Mun I | Nsb I | Psp1406 I | Van91 I | ||
17 | Bgl II | EcoT22 I | |||
18 | BciT130 I | Pst I | |||
19 | Sfi I | ||||
20 | Taq I | ||||
21 | Dpn I | ||||
22 | Aat II | ||||
23 | RspRS II |