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Experimental approaches involving Next-Generation Sequencing (NGS) have led to significant advancements in numerous research areas-for example, developmental biology and neuroscience-and are increasingly being applied toward the development of diagnostics and novel treatments for human disease. As the use of NGS advances in clinical and applied spaces, parallel processing of large numbers of samples, including single cells, will be critical. Takara Bio provides a powerful toolbox for analyzing the genomics of single cells. Our technologies enable research involving whole transcriptome analysis with single-cell or ultra-low-input RNA sequencing (SMART-Seq v4, SMART-Seq HT, and SMART-Seq v4 3' DE kits for high-quality samples, and the SMART-Seq Stranded Kit for both high- and low-quality samples) as well as variant analysis with single-cell whole genome amplification and sequencing (SMARTer PicoPLEX Gold kit). Our automation systems allow for scalability and simplification of single-cell library preparation. The SMARTer ICELL8 and ICELL8 cx microfluidic systems enable the rapid isolation and selection of single cells and the generation of libraries from selected cells using SMART-Seq chemistry. We will present product overviews and recent developments involving our single-cell NGS portfolio and applications on the SMARTer ICELL8 single-cell systems.
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