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ブレビバチルス分泌発現システム

使用文献

Brevibacillus Expression System

  • Cloning of a novel collagenase gene from the gram-negative bacterium Grimontia (Vibrio) hollisae 1706B and its efficient expression in Brevibacillus choshinensis.
    N. Teramura, K. Tanaka, K. Iijima, O. Hayashida, K. Suzuki, S. Hattori, S. Irie., (2011) J. Bacteriol. 193(12), 3049-3056.
    pNY326 DNAを用いたGrimontia (Vibrio) hollisae由来のCollagenase(約60 kDa)の分泌発現

  • Cloning, expression and purification of extracellular serine protease Esp, a biofilm-degrading enzyme, from Staphylococcus epidermidis.
    S. Sugimoto, T. Iwase, F. Sato, A. Tajima, H. Shinji, Y. Mizunoe., (2011) J. Appl. Microbiol., 111(6), 1406-1415.
    pNCOM2 DNAを用いたStaphylococcus epidermidis由来細胞外プロテアーゼ Esp (約27 kDa)の分泌発現

  • Brevibacillus expression system: host-vector system for efficient production of secretory proteins.
    M. Mizukami, H. Hanagata, A. Miyauchi., (2010) Curr. Pharm. Biotechnol., 11(3), 251-258.
    Brevibacillus expression systemに関する総説・使用例

  • Extracellular production of riboflavin-binding protein, a potential bitter inhibitor, by Brevibacillus choshinensis
    Maehashi K., Matano M., Saito M., Udaka S., (2010) Protein Expression and Purification, 71, 85-90.
    pNCMO2を用いたChicken由来riboflavin-binding protein(約30 kDa)の分泌発現

  • Expression and purification of canine granulocyte colony-stimulating factor (cG-CSF).
    A. Yamamoto, A. Iwata, T. Saito, F. Watanabe, S. Ueda., (2009) Vet. Immunol. Immunopathol., 130(3-4), 221-225.
    pNY326 DNAを用いたイヌ由来G-CSF(約18 kDa)の分泌発現

  • Histidine-regulated activity of M-ficolin
    Tanio M. and Kohno T., (2009) Biochem. J., 417, 485-491.
    pNCOMO2 DNAを用いたヒトM-フィコリン異物認識ドメイン(FD1) (26.8 KDa)の分泌発現

  • Amino acid-selective isotope labeling of proteins for NMR study: proteins secreted by Brevibacillus choshinensis
    Tanio M., Tanaka R., Tanaka T., and Kohno T., (2009) Analytical Biochemistry, 386, 156-160.
    ヒトFK506結合タンパク質(FKBP)およびヒトM-フィコリン異物認識ドメイン(FD1)のpNCMO2 DNAを用いた分泌発現時におけるアミノ酸選択的標識

  • 15N isotope labeling of a protein secreted by Brevibacillus choshinensis for NMR study
    Tanio M. Tanaka T. and Kohno T., (2008) Analytical Biochemistry, 373, 164-166.
    pNCOMO2 DNAを用いたヒトFK506結合タンパク質(約12 kDa)の分泌発現

参考文献

Brevibacillus Expression System
1) H. Takagi, K. Kadowaki and S. Udaka., (1989) Agric. Biol. Chem., 53 (3), 691-699.
2) T. Takano, A. Miyauchi, H. Takagi, K. Kadowaki, K. Yamane, S. Kobayashi., (1992) Biosci. Biotech. Biochem., 56 (5), 808-809.
3) H. Tojo, T. Asano, K. Kato, S. Udaka, R. Horinouchi, A. Kakinuma., (1994) J. Biotechnol., 33 (1), 55-62.
4) H. Yamagata, K. Nakahama, Y. Suzuki, A. Kakinuma, N. Tsukakoshi, S. Udaka., (1989) Proc. Natl. Acad. Sci. USA., 86, 3589-3593.
5) K. Yashiro, J. W. Lowenthal, T. E. O’ Neil, S. Ebisu, H. Takagi, (2001) Protein Expression and Purification, 23, 113-120.
6) H. Onishi, M. Mizukami, H. Hanagata, M. Tokunaga, T. Arakawa and A.Miyauchi., (2013) Protein Expression and Purification 91, 184–191.

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