In-Fusion® HD EcoDry™ Cloning Kit
使用文献
- In-Fusion PCR Cloning KitのBioBrickシステムへの利用
In-Fusion BioBrick assembly and re-engineering
S. C. Sleight, B. A. Bartley, J. A. Lieviant, and H. M. Sauro
Nucleic Acids Res. (2010) April; 1-13. - In-Fusionクローニングシステムを用いて、抗体可変領域のクローニングを迅速に行い、組換えモノクローナル抗体の発現に利用した例
A method for rapid, ligation-independent reformatting of recombinant monoclonal antibodies
Jones ML, Seldon T, Smede M, Linville A, Chin DY, Barnard R, Mahler SM, Munster D, Hart D, Gray PP, Munro TP
J Immunol Methods, (2010) Feb 354, 85–90. - In-Fusion Advantage PCR Cloning Kitの使用例
Ribosome stalk assembly requires the dual-specificity phosphatase Yvh1 for the exchange of Mrt4 with P0
Kai-Yin Lo, Zhihua Li, Feng Wang, Edward M. Marcotte, and Arlen W. Johnson
J. Cell Biol. (2009) Sep;186:849-862. - In-Fusion PCR Cloning Kitでターゲット配列に変異を導入した例
T-box 2, a mediator of Bmp-Smad signaling, induced hyaluronan synthase 2 and Tgfβ2 expression and endocardial cushion formation
Manabu Shirai, Kyoko Imanaka-Yoshida, Michael D. Schneider, Robert J. Schwartz, and Takayuki Morisaki
PNAS, (2009) Nov;106:18604-18609. - In-Fusion PCR Cloning Kitで計4断片でのプラスミド構築例
In-fusion assembly: seamless engineering of multidomain fusion proteins, modular vectors, and mutations.
B Zhu, G Cai, EO Hall, and GJ Freeman
Biotechniques, (2007) Sep;43(3):354-359.
参考文献
- Zhu, B. et al. (2007) BioTechniques 3:354-359.
- Marsischky, G. & LaBaer, J. (2004) Genome Res. 14:2020-2028.
- Hartman, S. et al. (January 2005) Clontechniques XX(1):26-27.
- Berrow, N.S. et al. (2007) Nucleic Acids Res. 35(6):e45.3.
