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Guide-it™ Recombinant Cas9 (Electroporation-Ready)

使用文献

Generation of Pax6-IRES-EGFP knock-in mouse via the cloning-free CRISPR/Cas9 system to reliably visualize neurodevelopmental dynamics
Yukiko U. Inoue, Yuki Morimoto, Mikio Hoshino, Takayoshi Inoue Neuroscience Research 2018 132: 1–7.

Electroporation of Cas9 protein/sgRNA into early pronuclear zygotes generates non-mosaic mutants in the mouse.
Hashimoto M, Yamashita Y and Takemoto T Dev Biol. 2016 Oct 1;418(1):1-9.

Somatic cell reprogramming-free generation of genetically modified pigs
Tanihara F, Takemoto T et al Science Advances 14 Sep 2016:Vol.2, no. 9, e1600803

Highly efficient genome editing for single-base substitutions using optimized ssODNs with Cas9-RNPs.
Okamoto, Sachiko, et al. Scientific Reports (2019) 9(1):4811.

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