Capturemは革新的な膜テクノロジーにより、室温下での迅速なタンパク質操作を可能にした画期的な製品です。

レジンカラムやビーズ法では煩雑な操作や長い操作時間などが、特にサンプル数が多い場合のデメリットでした。

長年タンパク質を扱う実験をされている方はもちろん、これから始める方にも操作の手間を大幅に短縮するCapturemをおススメします。

クローニングからタンパク質精製まで、タカラバイオでは多数の製品を取り揃えています。
以下に、実験操作の短縮につながる製品「Time Saving製品」をフローとともにご紹介します。

フローチャート検索

タンパク質実験の流れに合わせて各製品の検索が可能です。

Capturemとは捕獲（Capture）と膜（Membrane）を組み合わせた造語で、捕獲能力のあるメンブレンを意味します。

		Miniprep Column	96-well plate	24-well plate	Maxiprep Column	Large volume
タンパク質精製	Capturem His-Taggedシリーズ	✔	✔	✔	✔	✔
抗体精製・濃縮	Capturem Protein Aシリーズ	✔	✔	✔	✔
	Capturem Protein Gシリーズ	✔	✔	✔	✔
	Capturem Streptavidinシリーズ	✔	✔
免疫沈降・共免疫沈降	Capturem IP & Co-IP Kit	✔
抗体の分析	Capturem Trypsin (Mass Spectrometry Grade)	✔	✔
	Capturem Trypsin	✔
	Capturem Pepsin	✔
エキソソームなどの細胞外小胞（EV）精製	Capturem Extracellular Vesicle Isolation Kit	✔			✔
	Capturem Exosome Isolation Kit (Cell Culture)				✔

★ Capturemに関するQ＆Aはこちら

Capturemシリーズを使った実験フロー例

★ 無料サンプル配布中 ★

タンパク質精製（Hisタグ）

• A revolutionary solution for recombinant protein purification
• Simplified purification of active, secreted his-tagged proteins
• Rapid, high-purity isolation of his‑tagged proteins directly from large volumes of cell lysate and culture supernatant

抗体精製・濃縮

• Rapid screening of hybridoma clones for the best monoclonal antibody using Capturem Protein A technology
• Rapid and high-throughput purification of antibodies with Capturem Protein G
• Simple, rapid streptavidin-based enrichment using Capturem technology

免疫沈降・共免疫沈降

• Fast, efficient, and specific immunoprecipitation and co-immunoprecipitation with Capturem Protein A technology

抗体の分析

• Protein and whole-cell lysate digestion with Capturem Trypsin technology
• Complete antibody digestion with pepsin in just three minutes

抗体標識（ラべリング）

• わずか15分で完了する「Capturemテクノロジー」を用いた抗体標識

エキソソームなどの細胞外小胞（EV）精製

• Rapid, pure, and concentrated purification of extracellular vesicles from biofluids

Capturem His-Tagged Purificationシリーズ

• An anti-amoebic vaccine: generation of the recombinant antigen LC3 from E ntamoeba histolytica linked to mutated exotoxin A (PEΔIII) via the Pichia pastoris system.
  Martínez-Hernández S. L. et al., Biotechnol. Lett. (2017) 39, 1149-1157.
• Deciphering global gene expression and regulation strategy in Escherichia coli during carbon limitation.
  Li Z. et al., Microb Biotechnol. (2019) 12, 360-376.
• DELLA proteins negatively regulate dark-induced senescence and chlorophyll degradation in Arabidopsis through interaction with the transcription factor WRKY6.
  Zhang Y. et al., Plant Cell Rep. (2018) 37, 981-992.
• Evolution of (p)ppGpp-HPRT regulation through diversification of an allosteric oligomeric interaction.
  Anderson B. W. et al., eLife (2019) 8, e47534.
• Expanding the substrate scope of phenylacetone monooxygenase from Thermobifida fusca towards cyclohexanone by protein engineering.
  Guang Y. et al., Catalysis Communications (2019) 119, 159-163.
• Functional analysis of N-terminal propeptide in the precursor of Vibrio vulnificus metalloprotease by using cell-free translational system.
  Kawase T. et al., Protein Expr. Purif. (2018) 149, 13-16.
• Functional characterization of unique enzymes in Xanthomonas euvesicatoria related to degradation of arabinofurano-oligosaccharides on hydroxyproline-rich glycoproteins.
  Nakamura M. et al., PLoS One (2018) 13, e0201982.
• Functional identification of glutamate cysteine ligase and glutathione synthetase in the marine yeast Rhodosporidium diobovatum.
  Kong M. et al., Sci. Nat. (2018) 105, 4.
• Functional prediction and characterization of Dip2 gene in mice.
  Jun M. et al., Cell Biology International (2019) 43, 9.
• Novel mutations and a severe neurological phenotype in Sjögren-Larsson syndrome patients from Iran.
  Kariminejad A. et al., Eur. J. Med. Genet. (2018) 61, 139-144.
• Proteases, actinidin, papain and trypsin reduce oral biofilm on the tongue in elderly subjects and in vitro.
  Mugita N. et al., Arch. Oral Biol. (2017) 82, 233-240.
• The Egyptian rousette genome reveals unexpected features of bat antiviral immunity.
  Pavlovich S. S. et al., Cell (2018) 173, 1098-1100.e18.
• The endochitinase VDECH from Verticillium dahliae inhibits spore germination and activates plant defense responses.
  Cheng X.-X. et al., Plant Sci. (2017) 259, 12-23.
• Recombinant adenovirus carrying a core neutralizing epitope of porcine epidemic diarrhea virus and heat-labile enterotoxin B of Escherichia coli as a mucosal vaccine.
  Do V. T. et al., Arch. Virol. (2020) 165, 609-618.
• Age- and α-Synuclein-Dependent Degeneration of Dopamine and Noradrenaline Neurons in the Annual Killifish Nothobranchius furzeri.
  Matsui H. et al., Cell Reports (2019) 26, 1727-1733. e6

Capturem Protein Aシリーズ

• EFab domain substitution as a solution to the light-chain pairing problem of bispecific antibodies.
  Cooke H. A. et al., MAbs. (2018) 10, 1248-1259.
• Identification of a novel neutralizing and two non-neutralizing epitopes on Epstein-Barr virus gp350 protein.
  Mutsvunguma L. Z. et al., bioRxiv (2018) 302844.
• Identification of multiple potent neutralizing and non-neutralizing antibodies against Epstein-Barr virus gp350 protein with potential for clinical application and as reagents for mapping immunodominant epitopes.
  Mutsvunguma L. Z. et al., Virology (2019) 536, 1-15.

Capturem IP & Co-IP

• A novel protein L-cofilin2 identified in Lampetra morii with roles of immune response and cell proliferation promotion.
  Li Y. et al., Acta Biochim Biophys Sinica. (2019) 51, 1300-1303.
• Interplay between CTHRC1 and the SU protein of avian leukosis virus subgroup J (ALV-J) facilitates viral replication.
  Pang Y. et al., Virus Research (2019) 264, 32-39.
• Primary tumor‐secreted VEGF induces vascular hyperpermeability in premetastatic lung via the occludin phosphorylation/ubiquitination pathway.
  Li R. et al., Molecular Carcinogenesis (2019) 58(12), 2316-2326.
• Role of microtubule-associated protein 6 glycosylated with Gal-(β-1,3)-GalNAc in Parkinson's disease.
  Li M. et al., Aging. (2019) 11, 4597-4610.
• The intercellular expression of type-XVII collagen, laminin-332, and integrin-β1 promote contact following during the collective invasion of a cancer cell population.
  Kumagai Y. et al., Biochemical and Biophysical Research Communications (2019) 514, 1115-1121.

TALON resin
TALON Metal Affinity Resin

His60 Ni resin
His60 Ni Superflow Resin

Magnetic beads
TALON Magnetic Beads
His60 Ni Magnetic Beads

Cell lysis buffer
xTractor Buffer & Buffer Kit