次世代シーケンス(NGS)解析における長鎖Amplicon-sequence用酵素として最適
Long-range PCR in next-generation sequencing: comparison of six enzymes and evaluation on the MiSeq sequencer.
Haiying Jia, Yunfei Guo, Weiwei Zhao & Kai Wang, Scientific Reports (2014) 4:5737.doi:10.1038/srep05737
SETBP1, JAK3のExome Seaquenceに向けたExon領域の増幅に使用
Exome sequencing identifies secondary mutations of SETBP1 and JAK3 in juvenile myelomonocytic leukemia.
Sakaguchi, H., Okuno, Y., Muramatsu, H., Yoshida, K., Shiraishi, Y., Takahashi, M., Kon, A., Sanada, M., Chiba, K., Tanaka, H., Makishima, H., Wang, X., Xu, Y., Doisaki, S., Hama, A., Nakanishi, K., Takahashi, Y., Yoshida, N., Maciejewski, J. P., Miyano, S., Ogawa, S. & Kojima, S., Nat Genet, (2013) 45: 937-41.
Full-circle PCR法によるHPV16の増幅とNGSによる変異解析に使用
Genetic Variation of Human Papillomavirus Type 16 in Individual Clinical Specimens Revealed by Deep Sequencing.
Kukimoto, I., Maehama, T., Sekizuka, T., Ogasawara, Y., Kondo, K., Kusumoto-Matsuo, R., Mori, S., Ishii, Y., Takeuchi, T., Yamaji, T., Takeuchi, F., Hanada, K. & Kuroda, M. PLoS One. (2013) 8 :e80583.
Kimura Y., Kawasaki S., Yosh, J. Bacteriol,(2009) 10.1128/JB.01118-09.
Glycine betaine biosynthesized from glycine provides an osmolyte for cell growth and spore germination during osmotic stress in Myxococcus xanthus.
imoto H., and Takegawa K.,
Triple N-Glycosylation in the Long S5-P Loop Regulates the Activation and Trafficking of the Kv12.2 Potassium Channel.
Noma K., Kimura K., Minatohara K., Nakashima H., Nagao Y., Mizoguchi A., and Fujiyoshi Y., J. Biol. Chem, (2009) 284:33139-33150.
Identification and Characterization of R2R3-MYB and bHLH Transcription Factors Regulating Anthocyanin Biosynthesis in Gentian Flowers.
Nakatsuka T., Haruta K.S., Pitaksutheepong C., Abe Y., Kakizaki Y., Yamamoto K., Shimada N., Yamamura S., and Nishihara M., Plant Cell Physiol, (2008) 49:1818-1829.
Efficient transfer of base changes from a vector to the rice genome by homologous recombination:involvement of heteroduplex formation and mismatch correction.
Johzuka-Hisatomi Y, Terada R, and Iida S., Nucleic Acids Res, (2008) 36:4727-4735.