SP6 RNA PolymeraseによるRNA合成反応
| 10×SP6 RNA Polymerase Buffer * | 2 μl |
| 100 mM DTT * | 2 μl |
| 0.1% BSA * | 2 μl |
| ATP、CTP、GTP、UTP ** | each 0.5 mM |
| RNase Inhibitor | 20 U |
| Template DNA | 50 ～ 500 ng |
| SP6 RNA Polymerase | 10 ～ 50 U |
| 滅菌蒸留水 | up to 20 μl |
| 10×SP6 RNA Polymerase Buffer * | 2 μl |
| 100 mM DTT * | 2 μl |
| 0.1% BSA * | 2 μl |
| ATP、CTP、GTP、UTP ** | each 0.5 mM |
| RNase Inhibitor | 20 U |
| Template DNA | 50 ～ 500 ng |
| SP6 RNA Polymerase | 10 ～ 50 U |
| 滅菌蒸留水 | up to 20 μl |