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Tissue | Tested Cell Lines | Cell Lines Origin | Remarks |
---|---|---|---|
Brain | BV2 | immortalized murine primary microglial cells | Coat with Fibronectin: 1 μg/cm2 |
Breast | MCF-7 | pleural effusion of metastatic human breast adenocarcinoma | Coat with Fibronectin: 1 μg/cm2 |
Colon | HT-29 | human colon adenocarcinoma | Coat with Vitronectin: 0.5 μg/cm2 |
Connective tissue | HT1080 | human fibrosarcoma | Coat with Fibronectin: 1 μg/cm2 |
Liver | HepG2 | hepatocellular carcinoma of the human liver | Coat with Vitronectin: 0.5 μg/cm2 |
Lung | A-549 | human lung carcinoma | Coat with Vitronectin: 0.5 μg/cm2 |
Prostate | LNCaP | lymph node metastasis of human prostate adenocarcinoma | 3D Tumorsphere Medium XF(製品コード C-28070)での3次元培養を推奨 |
Primary Cancer Culture System | 3D Tumorsphere Medium XF | Cancer Cell Line Medium XF | ||
Main application | Main application | Primary cancer cell isolation/maintenance of heterogeneity | Extended 3D tumorsphere culture of cancer cell lines/enrichment of CSCs | Adherent long-term expansion of cancer cell lines |
Culture pattern | 2D/3D | 3D | 2D | |
Formulation | Defined formulation, animal-component free | Serum-free, xeno-free | Serum-free, xeno-free | |
Additional supplementation required | No | No | No | |
Plasticware | TC-treated | Suspension culture | TC-treated | |
Culture surface treatment required | NCCD-reagent (incl.) | No | Human Fibronectin | |
Product Application | Slective isolation of malignant cells from primary tumors | ✔ | × | × |
Slective isolation of malignant cells from PDX | ✔ | × | × | |
Limited stroma support | ✔ | × | × | |
Prevention of stroma overgrowth | ✔ | × | × | |
Enrichment of malignant cells from established culture | ✔ | × | × | |
Depletion of non-cancerous cells from established cultures | ✔ | × | × | |
Serial 3D tumorsphere culture of established cancer cell lines | × | ✔ | × | |
Serial 3D mammosphere culture of established cancer cell lines | × | ✔ | × | |
Serial 3D neurosphere culture of established cancer cell lines | × | ✔ | × | |
Mammosphere culture(primary neural stem cells) | × | ✔ | × | |
Neurosphere culture(primary neural stem cells) | × | ✔ | × | |
Adherent culture of established cancer cell lines | × | × | ✔ | |
Establish cell lines from primary cancer cells isolated with PCCS | × | ✔ | ✔ |