Guide-it™ sgRNA In Vitro Transcription and Screening System
使用文献
- 遺伝子変異マウス作製時の変異確認のためにGuide-it Complete sgRNA Screening Systemを使用
Exome sequencing in the knockin mice generated using the CRISPR/Cas system
Kazuo Nakajima, An-a Kazuno, John Kelsoe, Moe Nakanishi, Toru Takumi ＆ Tadafumi Kato
Scientific Reports 6, Article number: 34703 (2016) - ヒトiPS細胞の高効率なゲノム編集「1塩基置換導入法」に必要なシングルセルクローニング工程に、Cas9タンパク質とsgRNAの複合体（RNP）を使用
Highly efficient genome editing for single-base substitutions using optimized ssODNs with Cas9-RNPs.
Okamoto, Sachiko, et al. Scientific Reports (2019) 9(1):4811.
