神経幹細胞維持／神経分化培地 RHB-A^®

ヒト・マウス神経幹（NS）細胞の維持：

Nestin	Vimentin
3CB2	Map 2
マウスNS細胞

ヒトNS細胞

ヒト・マウス神経幹（NS）細胞の神経細胞への分化：

マウスES細胞の神経前駆細胞への分化：

1. フィーダーフリーの継代数の低いES細胞を0.5～2×10⁴ cells/cm²の密度でゼラチンコートのプレート^＊にRHB-A培地を用いて播種する。
2. 1～2日ごとに培地を交換する。神経細胞への分化の初期段階でかなりの数の死細胞が観察される。
3. 神経細胞への分化を形態観察、またマーカーの発現などで観察する神経細胞への分化は、4～6日目から観察され、7～9日目にニューロン成熟が見られる。

1. Veliparib in combination with radiotherapy for the treatment of MGMT unmethylated glioblastoma
   Jue TR et al. (2017) Mar; J. Transl. Med. 15(1) doi:10.1186/s12967-017-1164-1.
2. Combination of palbociclib and radiotherapy for glioblastoma
   Whittaker S et al. (2017) Jul; Cell. Death. Descov. 3 doi: 10.1038/cddiscovery.2017.33.

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3. Adherent Neural Stem (NS) cells from fetal and adult forebrain.
   Pollard SM, Conti L, Sun Y, Goffredo D, Smith A (2006) Cerebral Cortex 16:112-120.
4. Conversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture.
   Ying QL, Stavridis M, Griffiths D, Li M, Smith A (2003). Nature Biotechnology 21:183-186.
5. Fibroblast growth factor induces a neural stem cell phenotype in foetal forebrain progenitors and during embryonic stem cell differentiation.
   Pollard SM, Wallbank R, Tomlinson S, Grotewold L, Smith A (2008) Molecular and Cellular Neuroscience 38:393-403.
6. Glioma stem cell lines expanded in adherent culture have tumor‐specific phenotypes and are suitable for chemical and genetic screens.
   Pollard SM, Yoshikawi K, Clarke ID, Danovi D, Stricker S, Russell R, Bayani J, Head R, Lee M, Bernstein M, Squire J, Smith A, Dirks P (2009). Cell Stem Cell 4:568-580.
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   Diogo MM, Henrique D, Cabral JM (2008) Biotechnology and Applied Biochemistry 49:105-112.
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   Abranches E, Silva M, Pradier L, Schulz H, Hummel O, Henrique D, Bekman E (2009) PLoS ONE 4 (7): e6286
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   Hansen DV, Lui JH, Parker PRL, Kriegstein AR (2010) Nature 464(7288):554-561.
10. Non-immortalized human neural stem (NS) cells as a scalable platform for cellular assays.
    Hook L, Vives J, Fulton N, Leveridge M, Lingard S, Bootman MD, Falk A, Pollard SM, Allsopp TE, Dalma-Weiszhausz D, Tsukamoto A, Uchida N, Gorba T. Neurochem Int. (2011) Sep;59(3):432-444.