S1 Nuclease | Mung Bean Nuclease |
BAL 31 Nuclease | Exonuclease I | |
---|---|---|---|---|
反応特異性 | endonuclease ssDNA, ssRNA |
endonuclease ssDNA, ssRNA |
endonuclease ssDNA exonuclease 5'→3', 3'→5'dsDNA |
exonuclease 3'→5'ssDNA |
最終生成物 | 5'-P mononucleotide 5'-P oligonucleotide |
5'-P mononucleotide 5'-P oligonucleotide |
5'-P mononucleotide | 5'-P mononucleotide |
分子量 | 32,000 | 39,000 | 73,000~83,000 | 52,000 |
至適pH | 4.5 | 5.0 | 8.1 | 9.5 |
金属イオン | Zn2+ | Zn2+ | Ca2+, Mg2+ | Mg2+ |
Exonuclease III | Recombinant DNase I | Ribonuclease H | Micrococcal Nuclease | |
---|---|---|---|---|
反応特異性 | exonuclease 3'→5'dsDNA |
endonuclease ssDNA, dsDNA |
RNA-DNA hybrid 特異的 endonuclease |
endonuclease ssDNA, ssRNA dsDNA |
最終生成物 | ssDNA 5'-P mononucleotide |
5'-P oligonucleotide | ssDNA 5'-P oligonucleotide |
3'-P mononucleotide 3'-P oligonucleotide |
分子量 | 28,000 | 39,000 | 21,000 | 16,800 |
至適pH | 8.0 | 7.0~8.0 | 8.0 | 9.2 |
金属イオン | Mg2+ | Mg2+, Mn2+ | Mg2+, Mn2+ | Ca2+ |