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Faustovirus Capping Enzyme (S17) (25 U/μl) | 500 U |
10×Capping Buffer 2 | 100 μl |
S-adenosylmethionine (SAM) (32 mM) | 100 μl |
GTP (10 mM) | 50 μl |
1× | Capping Buffer 2 |
25 μM | GTP |
20 μCi/ml | [3H]SAM |
2.5 μg/50 μl | 100 nt RNA |
RNA transcript (50 μg) *1 | 38 μl |
10×Capping Buffer 2 | 5 μl |
GTP(10 mM) | 2.5 μl |
SAM(2 mM, dilute 32 mM stock to 2 mM)*2 | 2.5 μl |
Faustovirus Capping Enzyme (S17)(25 U/μl)*3 | 2 μl |
Total | 50 μl*4 |
RNA transcript (50 μg) *1 | 34 μl |
10×Capping Buffer 2*5 | 5 μl |
GTP(10 mM) | 2.5 μl |
SAM(4 mM, dilute 32 mM stock to 4 mM)*2 | 2.5 μl |
Faustovirus Capping Enzyme (S17)(25 U/μl)*3 | 2 μl |
mRNA Cap 2’-O-Methyltransferase(50 U/μl)*3 | 4 μl |
Total | 50 μl*4 |
mRNA合成(in vitro Transcription)製品ガイド
mRNA Cap 2'-O-Methyltransferase
RNase-free Water
Takara IVTpro™ mRNA Synthesis System
Template Vector (BspQ I) for T7 mRNA Synthesis
PrimeCap™ T7 RNA Polymerase (low dsRNA)
T7 RNA Polymerase, HQ
T7 RNA Polymerase ver.2.0
Pyrophosphatase (inorganic), HQ
Pyrophosphatase (inorganic)
BspQ I, HQ
BspQ I
Recombinant RNase Inhibitor ver.2.0
dsRNAの生成を大幅に低減し、高品質なmRNAの調製が可能
m7G capped RNA(Cap 0)をCap 1構造にするメチル基転移酵素
UMI搭載!マウスBCR H鎖/L鎖 V(D)J可変領域の完全長シーケンスが可能
高感度で使いやすいデジタルPCRシステム
オールインワン、7色検出のデジタルPCRシステム
RNAの5’末端にm7G cap構造(Cap 0)を付加する酵素