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Linearized Template Vector (BspQ I)(50 ng/μl) | 10 μl |
FLuc Control Fragment (BspQ I)(100 ng/μl)*1 | 10 μl |
5×In-Fusion Snap Assembly Master Mix*2 | 20 μl |
10×Transcription Buffer | 40 μl |
10×ATP | 40 μl |
10×CTP | 40 μl |
10×GTP | 40 μl |
10×UTP | 40 μl |
10×Enzyme Mix 2 | 40 μl |
Nuclease-Free Water | 1 ml×3 |
DNase I | 80 μl |
Lithium Chloride Precipitation Solution | 600 μl |
Positive Control Template (FLuc)(0.5 μg/μl)*3 | 10 μl |
IVT反応例:Takara IVTpro T7 mRNA Synthesis Kit (low dsRNA)(製品コード 6134)
ベクター情報:Linearized Template Vector (BspQ I)
mRNA合成(in vitro Transcription)製品ガイド
Template Vector (BspQ I) for T7 mRNA Synthesis
BspQ I
Takara Stable Competent Cells
Ribonuclease Inhibitor
Recombinant RNase Inhibitor ver.2.0
Ribonucleoside 5'-Triphosphates
Recombinant DNase I (RNase-free)
Poly(A) Polymerase
T7 RNA Polymerase ver.2.0
PrimeCap™ T7 RNA Polymerase (low dsRNA)
Faustovirus Capping Enzyme (S17)
Vaccinia Capping Enzyme
mRNA Cap 2'-O-Methyltransferase
Pyrophosphatase (inorganic)
Human Cell-Free Protein Expression System
In-Fusion® Snap Assembly Master Mix
RNase-OFF®
mRNA作製
dsRNAの生成を大幅に低減し、高品質なmRNAの調製が可能
Poly(A) 配列後に余分な配列を持たない“scarless”なmRNA調製に
in vitro転写による高品質、高収量なCleanCap修飾mRNA合成キット
反応性を飛躍的に高めたバージョンアップ品
従来酵素に比べ幅広い温度帯で反応可能なm7G cap構造(Cap 0)付加酵素