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1× | Capping Buffer 2, HQ | |
500 μM | GTP | |
100 μM | SAM | |
1 μg/20 μl | 100 nt RNA |
RNA transcript (50 μg)*1 | 38 μl |
10× Capping Buffer 2, HQ | 5 μl |
GTP(10 mM) | 2.5 μl |
SAM(2 mM, dilute 32 mM stock to 2 mM)*2 | 2.5 μl |
Faustovirus capping enzyme (S17), HQ (25 U/μl)*3 | 2 μl |
Total | 50 μl*4 |
RNA transcript (50 μg) *1 | 34 μl |
10× Capping Buffer 2, HQ | 5 μl |
GTP(10 mM) | 2.5 μl |
SAM(4 mM, dilute 32 mM stock to 4 mM)*2 | 2.5 μl |
Faustovirus Capping Enzyme (S17), HQ(25 U/μl)*3 | 2 μl |
mRNA Cap 2’-O-Methyltransferase, HQ(50 U/μl)*3 | 4 μl |
Total | 50 μl*4 |
mRNA合成(in vitro Transcription)製品ガイド
mRNA製造用酵素の品質グレードとは?(RUO/HQ/GMP)
mRNA Cap 2'-O-Methyltransferase, HQ
PrimeCap™ T7 RNA Polymerase (low dsRNA), HQ
T7 RNA Polymerase, HQ
Pyrophosphatase (inorganic), HQ
BspQ I, HQ
Faustovirus Capping Enzyme (S17)
mRNA Cap 2'-O-Methyltransferase
PrimeCap™ T7 RNA Polymerase (low dsRNA)
T7 RNA Polymerase ver.2.0
Pyrophosphatase (inorganic)
Recombinant RNase Inhibitor ver.2.0
BspQ I
Takara IVTpro™ mRNA Synthesis System (low dsRNA)
Takara IVTpro™ mRNA Synthesis System
Ribonucleoside 5'-Triphosphates
RNase-free Water
従来酵素に比べ幅広い温度帯で反応可能なm7G cap構造(Cap 0)付加酵素
組織中の形態学的位置情報とリンクした遺伝子発現データを取得します。
m7G capped RNA(Cap-0)をCap-1構造に変換するメチル基転移酵素(High Quality grade)
調製済みの反応液でさらに簡便に!検便検査キット(EHEC、サルモネラ属菌/赤痢菌)
dsRNAの生成を大幅に低減し、高品質mRNAを合成可能(High Quality grade)