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1× | Capping Buffer 2, HQ | |
20 μM | SAM | |
1 μg/20 μl | 100 nt Cap-0 RNA |
Cap-0 RNA (50 μg) *1 | 40 μl |
10× Capping Buffer 2, HQ | 5 μl |
SAM(4 mM, dilute 32 mM stock to 4 mM)*2 | 2.5 μl |
mRNA Cap 2’-O-Methyltransferase, HQ (50 U/μl)*3 | 2.5 μl |
Total | 50 μl*4 |
RNA transcript (50 μg) *1 | 34 μl |
10× Capping Buffer 2, HQ | 5 μl |
GTP(10 mM) | 2.5 μl |
SAM(4 mM, dilute 32 mM stock to 4 mM)*2 | 2.5 μl |
Faustovirus Capping Enzyme (S17), HQ(25 U/μl)*3 | 2 μl |
mRNA Cap 2’-O-Methyltransferase, HQ(50 U/μl)*3 | 4 μl |
Total | 50 μl*4 |
mRNA合成(in vitro Transcription)製品ガイド
mRNA製造用酵素の品質グレードとは?(RUO/HQ/GMP)
カスタム製造のご案内
Faustovirus Capping Enzyme (S17), HQ
PrimeCap™ T7 RNA Polymerase (low dsRNA), HQ
T7 RNA Polymerase, HQ
Pyrophosphatase (inorganic), HQ
Recombinant RNase Inhibitor, HQ
BspQ I, HQ
Faustovirus Capping Enzyme (S17)
mRNA Cap 2'-O-Methyltransferase
PrimeCap™ T7 RNA Polymerase (low dsRNA)
T7 RNA Polymerase ver.2.0
Pyrophosphatase (inorganic)
Recombinant RNase Inhibitor ver.2.0
BspQ I
Takara IVTpro™ mRNA Synthesis System (low dsRNA)
Takara IVTpro™ mRNA Synthesis System
Ribonucleoside 5'-Triphosphates
RNase-free Water
mRNAワクチン研究/RNA医薬開発のスケールアップ・プロセス開発等に利用可能
dsRNAの生成を大幅に低減し、高品質mRNAを合成可能(High Quality grade)
幅広い温度帯で反応可能なm7G cap構造(Cap-0)付加酵素(High Quality grade)
くるみ・アーモンドなどのふき取り検査に